Spatial localization of cardiac optical mapping with multiphoton excitation.
Spatial localization of cardiac optical mapping with multiphoton excitation.
复制标题
多光子激发心脏光学测绘的空间定位。
DOI：
10.1117/1.1559831
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发表时间：
2003
影响因子：
3.5
通讯作者：
Knisley,StephenB
中科院分区：
文献类型：
--
作者：
Ramshesh,VenkatK;Knisley,StephenB
通过激光束的心脏光学标测询问的区域的深度和半径取决于光子在心脏内的行进。限制询问的深度和半径范围将是有用的。我们模拟了聚光透镜将激光集中在心脏内目标深度的效果，以及近红外激发以增加穿透并产生双光子吸收的效果。蒙特卡罗模拟，包括一个0.55-NA的透镜，和吸收和散射的1064或488-nm的激光在3-D心脏组织中表示的组织内的激发通量的分布。结合跨膜电压敏感荧光（波长669 nm）的吸收和散射的后续模拟指示离开组织表面的荧光光子起源的位置。结果表明，与现有的心脏光学标测相比，心脏中高达300 μm深度的标测可以显著改善定位。所估计的询问区域足够小以在细胞或亚细胞尺度上检查心脏事件，并且可以允许在心脏中的各种深度处进行标测。©2003年摄影光学仪器工程师协会。
Depth and radius of regions interrogated by cardiac optical mapping with a laser beam depend on photon travel inside the heart. It would be useful to limit the range of depth and radius interrogated. We modeled the effects of a condensing lens to concentrate laser light at a target depth inside the heart, and near infrared excitation to increase penetration and produce two-photon absorption. A Monte Carlo simulation that incorporated a 0.55-NA lens, and absorption and scattering of 1064- or 488-nm laser light in 3-D cardiac tissue indicated the distribution of excitation fluence inside the tissue. A subsequent simulation incorporating absorption and scattering of transmembrane voltage-sensitive fluorescence (wavelength 669 nm) indicated locations from which fluorescence photons exiting the tissue surface originated. The results indicate that mapping at depths up to 300 μm in hearts can provide significant improvement in localization over existing cardiac optical mapping. The estimated interrogation region is sufficiently small to examine cardiac events at a cellular or subcellular scale and may allow mapping at various depths in the heart. ©2003 Society of Photo-Optical Instrumentation Engineers.