The influence of the ectomycorrhizal fungus Rhizopogon subareolatus on growth and nutrient element localisation in two varieties of Douglas fir (Pseudotsuga menziesii var. menziesii and var. glauca) in response to manganese stress.
The influence of the ectomycorrhizal fungus Rhizopogon subareolatus on growth and nutrient element localisation in two varieties of Douglas fir (Pseudotsuga menziesii var. menziesii and var. glauca) in response to manganese stress.
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DOI：
10.1007/s00572-008-0174-5
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发表时间：
2008-07
期刊：
影响因子：
3.9
通讯作者：
Polle, Andrea
中科院分区：
文献类型：
--
作者：
Ducic, Tanja;Parlade, Javier;Polle, Andrea
森林生态系统的酸化导致植物对微量元素锰（Mn）的可利用性增加，而锰在过量吸收时是有毒的。为了研究外生菌根是通过改善植物生长和营养还是通过将过量的Mn保留在菌丝被膜中来防止过量Mn的产生，本研究在沙地培养中，用外生菌根真菌Rhizopogon subareolatus对两个花旗松（menziesii pseudosuga menziesii）种群（DFM）和glauca （DFG）两个品种的幼苗进行了接种。接种5个月后，接种和未接种的一半幼苗在营养液中暴露过量Mn，再持续5个月。在这一时期结束时，评估了植物生产力、养分浓度、锰吸收和亚细胞分区化。未接种、未受胁迫的DFM植物产生的生物量比同样处理的DFG多2.5倍。营养液中过量的Mn导致针叶和根中Mn的大量积累，但生物量的损失很小。尽管菌根对植物磷营养有积极作用，但微孢子菌定殖对DFM的生长有轻微抑制，但对DFG的生长有显著降低（- 50%）。接种的花旗松幼苗生长下降是出乎意料的，因为菌根化程度不高，在DFM中约为30%，在DFG中约为8%。未阻止接种苗对高锰的积累。菌根根尖的菌丝被膜积累了二价阳离子，如Ca，而不是Mn，因此不提供一个屏障，以防止过量的Mn吸收到与小乳霉有关的植物中。
Acidification of forest ecosystems leads to increased plant availability of the micronutrient manganese (Mn), which is toxic when taken up in excess. To investigate whether ectomycorrhizas protect against excessive Mn by improving plant growth and nutrition or by retention of excess Mn in the hyphal mantle, seedlings of two populations of Douglas fir (Pseudotsuga menziesii), two varieties, one being menziesii (DFM) and the other being glauca (DFG), were inoculated with the ectomycorrhizal fungus Rhizopogon subareolatus in sand cultures. Five months after inoculation, half of the inoculated and non-inoculated seedlings were exposed to excess Mn in the nutrient solution for further 5 months. At the end of this period, plant productivity, nutrient concentrations, Mn uptake and subcellular compartmentalisation were evaluated. Non-inoculated, non-stressed DFM plants produced about 2.5 times more biomass than similarly treated DFG. Excess Mn in the nutrient solution led to high accumulation of Mn in needles and roots but only to marginal loss in biomass. Colonisation with R. subareolatus slightly suppressed DFM growth but strongly reduced that of DFG (−50%) despite positive effects of mycorrhizas on plant phosphorus nutrition. Growth reductions of inoculated Douglas fir seedlings were unexpected since the degree of mycorrhization was not high, i.e. ca. 30% in DFM and 8% in DFG. Accumulation of high Mn was not prevented in inoculated seedlings. The hyphal mantle of mycorrhizal root tips accumulated divalent cations such as Ca, but not Mn, thus not providing a barrier against excessive Mn uptake into the plants associated with R. subareolatus.
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影响因子：
9.4
作者：
DOSSKEY, MG;BOERSMA, L;LINDERMAN, RG
通讯作者：
LINDERMAN, RG
影响因子：
2.1
作者：
BETHLENFALVAY, GJ;FRANSON, RL
通讯作者：
FRANSON, RL
影响因子：
4.3
作者：
DAVIES, FT;POTTER, JR;LINDERMAN, RG
通讯作者：
LINDERMAN, RG
影响因子：
9.4
作者：
ARINES, J;VILARINO, A;SAINZ, M
通讯作者：
SAINZ, M
影响因子：
9.4
作者：
Eltrop, L;Marschner, H
通讯作者：
Marschner, H