Susceptibility of porcine IPI-2I intestinal epithelial cells to infection with swine enteric coronaviruses
Susceptibility of porcine IPI-2I intestinal epithelial cells to infection with swine enteric coronaviruses
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猪IPI-2I肠上皮细胞对猪肠道冠状病毒感染的敏感性
DOI：
10.1016/j.vetmic.2019.04.014
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发表时间：
2019-06-01
影响因子：
3.3
通讯作者：
Xiao, Ishaobo
中科院分区：
文献类型：
--
作者：
Wang, Xunlei;Fang, Liurong;Xiao, Ishaobo
猪肠冠状病毒(CoV)是引起仔猪腹泻的重要病原体。至少已鉴定出四种猪肠道冠状病毒，包括传染性胃肠炎病毒(TGEV)、猪流行性腹泻病毒(PEDV)、猪DeltaCoronaVirus(PDCoV)和新出现的类似HKU2的猪肠道甲型冠状病毒(PEAV)。小肠，特别是空肠和回肠，是这四种冠状病毒在体内最常见的靶标，临床感染的猪经常被这四种冠状病毒合并感染。本研究旨在探讨猪回肠上皮细胞系IPI-2I对不同猪肠冠状病毒的敏感性。我们发现IPI-2i细胞对TGEV、PDCoV和PEAV高度敏感，细胞病变效应和病毒增殖证明了这一点。然而，只有少数细胞可以被PEDV感染，这可能是由于IPI-2I细胞的异质性所致。从IPI-2I细胞亚克隆获得的同源细胞系IPI-FX对PEDV高度敏感。此外，IPI-FX细胞对TGEV、PDCoV和PEAV也高度敏感。因此，亚克隆的IPI-FX细胞系是研究猪肠道冠状病毒感染机制，特别是混合感染的理想细胞模型。
Swine enteric coronavirus (CoV) is an important group of pathogens causing diarrhea in piglets. At least four kinds of swine enteric CoVs have been identified, including transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and the emerging HKU2-like porcine enteric alphacoronavirus (PEAV). The small intestines, particularly the jejunum and ileum, are the most common targets of these four CoVs in vivo, and co-infections by these CoVs are frequently observed in clinically infected pigs. This study was conducted to investigate the susceptibility of the porcine ileum epithelial cell line, IPI-2I, to different swine enteric CoVs. We found that IPI-2I cells are highly susceptible to TGEV, PDCoV, and PEAV, as demonstrated by cytopathic effect and virus multiplication. However, only a small number of cells could be infected by PEDV, possibly due to the heterogeneity of IPI-2I cells. A homogeneous cell line, designated IPI-FX, obtained from IPI-2I cells by sub-cloning with limited serial dilutions, was found to be highly susceptible to PEDV. Furthermore, IPI-FX cells were also highly susceptible to TGEV, PDCoV, as well as PEAV. Thus, this sub-cloned IPI-FX cell line is an ideal cell model to study the mechanisms of infection, particularly co-infections of swine enteric CoVs.