Amphibian thrombocyte-derived extracellular vesicles, including microRNAs, induce angiogenesis-related genes in endothelial cells.
Amphibian thrombocyte-derived extracellular vesicles, including microRNAs, induce angiogenesis-related genes in endothelial cells.
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两栖动物血小板衍生的细胞外囊泡，包括 microRNA，在内皮细胞中诱导血管生成相关基因。
DOI：
10.1111/gtc.12882
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发表时间：
2021
期刊：
影响因子：
2.1
通讯作者：
Kayano Toume
中科院分区：
文献类型：
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作者：
Kenkichi Sugimoto;Kayano Toume
血小板在非哺乳类脊椎动物的血液中循环并参与止血;然而，血小板的许多详细特征仍不清楚。最近，我们建立了两栖类血小板细胞系。在这里，我们报告了血小板产生整合素α IIb（CD 41）阳性细胞外囊泡（EV）的发现，其中包括microRNA（miR）。流式细胞仪分析显示，CD 41+和磷脂酰丝氨酸在EV表面的表达。纳米跟踪分析显示，这些CD 41 + EV的直径约为100 nm。由于在非洲爪蛙中也观察到了CD 41 + EV，因此CD 41 + EV的产生可能在两栖动物中很常见。微阵列分析显示，CD 41 +EVs中含有多种miRs。这些CD 41 + EV被内皮细胞和巨噬细胞吞噬。qPCR分析显示，许多血管生成相关基因在CD 41 +EV处理的内皮细胞中上调。CD 41 + EV中某些miR的过表达增加了内皮细胞的增殖。这些结果表明，血小板产生CD 41 + EV，包括miR，其被内皮细胞接收以诱导血管生成相关基因的表达。这些结果表明，由血小板产生的CD 41 +EVs作为信号分子修复受损的血管。
Thrombocytes circulate in the blood of nonmammalian vertebrates and are involved in hemostasis; however, many detailed characteristics of thrombocytes remain unclear. Recently, we established an amphibian thrombocyte cell line. Here, we report the finding that thrombocytes produce integrin alpha IIb (CD41)‐positive extracellular vesicles (EVs), which include microRNAs (miRs). Flow cytometric analysis showed the expression of CD41+and phosphatidylserine on the surface of EVs. Nanotracking analysis showed that these CD41+EVs were approximately 100 nm in diameter. As CD41+EVs were also observed from African clawed frogs, the production of CD41+EVs might be common to amphibians. Microarray analysis showed that the CD41+EVs contain many kinds of miRs. These CD41+EVs were phagocytosed by endothelial cells and macrophages. qPCR analysis showed that many angiogenesis‐related genes were up‐regulated in CD41+EV‐treated endothelial cells. Over‐expression of some miRs in the CD41+EVs increased the proliferation of endothelial cells. These results indicated that thrombocytes produced CD41+EVs, including miRs, that were received by endothelial cells to induce the expression of angiogenesis‐related genes. These results indicated that the CD41+EVs produced from thrombocytes act as signaling molecules to repair damaged blood vessels.