CUT&tag-seq analysis of heat stress response in broiler liver provides novel insights into the improved thermotolerance by dietary phloretin
CUT&tag-seq analysis of heat stress response in broiler liver provides novel insights into the improved thermotolerance by dietary phloretin
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DOI：
10.1016/j.aoas.2023.02.002
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发表时间：
2023-02-24
影响因子：
5.9
通讯作者：
Pan, Hongbin
中科院分区：
文献类型：
--
作者：
Hu, Hong;Chen, Liang;Pan, Hongbin
本研究旨在通过靶下切割和标签片段化结合高通量测序（CUT&Tag-seq）技术研究根皮素通过核因子红细胞2相关2（Nrf 2）-DNA相互作用对热应激肉鸡肝脏抗氧化谱的影响。选用22日龄爱拔益加肉鸡160只，随机分为4组：常温组（NT：22.8-23.4 ℃和46- 55.8%湿度）饲喂基础日粮和三个高温组（中国夏季条件：29.7-31.7 ℃和77.9- 94.8%湿度）饲喂添加0（HT组）、100 mg/kg（LT组）和200 mg/kg（PT组）根皮素的基础日粮。肝脏样品取自42日龄的肉鸡。高温条件下增加（P
This work aimed to study the effect of phloretin on antioxidant profiles through nuclear factor erythroid 2- related 2 (Nrf2)-DNA interactions in the liver of heat-stressed broilers by Cleavage Under Targets and Tagmentation combined with high-throughput sequencing (CUT&Tag-seq). One hundred and sixty 22-day-old Arbor Acres broilers were divided into four groups: a normal temperature group (NT: 22.8-23.4 degrees C and 46-55.8 % humidity) was fed a basal diet and three high temperature groups (China Summer condition: 29.7-31.7 degrees C and 77.9-94.8 % humidity) were fed the basal diet supplemented with 0 (HT group), 100 mg/kg (LT group) and 200 mg/kg (PT group) phloretin. Liver samples were taken from 42-day-old broilers. High tem-perature condition increased (P