The vitellogenin cDNA of Cherax quadricarinatus encodes a lipoprotein with calcium binding ability, and its expression is induced following the removal of the androgenic gland in a sexually plastic system
The vitellogenin cDNA of Cherax quadricarinatus encodes a lipoprotein with calcium binding ability, and its expression is induced following the removal of the androgenic gland in a sexually plastic system
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DOI：
10.1016/s0016-6480(02)00053-9
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发表时间：
2002-07-01
影响因子：
2.7
通讯作者：
Sagi, A
中科院分区：
文献类型：
--
作者：
Abdu, U;Davis, C;Sagi, A
十足类甲壳动物卵母细胞成熟是一个分两步进行的过程。初级卵黄发生之后是一个可变的间歇期，一直持续到次级卵黄发生的开始，这是以卵黄蛋白在卵母细胞中快速积累为标志的。我们已经克隆了一个完整的红樱桃卵黄蛋白原基因。测序后的基因包含一个2584个氨基酸的开放阅读框，与其他甲壳类动物的卵黄蛋白原序列具有相似性。该信使核糖核酸编码至少两种先前发现的卵黄蛋白成分，表明初级翻译产物受到翻译后修饰，包括蛋白水解性切割。靠近mRNA 3‘端的区域编码一个先前特征的带负电荷的蛋白质(暂时命名为p(106))。我们在这里表明，p(106)的负电荷可能是由于它结合钙的能力所致。Northern杂交数据表明，该基因以8000个核苷酸转录本的形式表达，并且存在于次级卵黄生成雌性的肝胰腺中。雄性和雌性动物的初级卵黄生成和其他组织检查均为阴性。在两性动物中，去掉雄性腺体会导致卵黄蛋白原转录，这表明该基因受到雄性腺体的负调控。(C)2002年埃尔塞维尔科学公司(美国)。版权所有。
Oocyte maturation in decapod crustaceans is a two step process. Primary vitellogenesis is followed by a variable hiatus that lasts up to the onset of secondary vitellogenesis, which is marked by the rapid accumulation of yolk proteins in the oocytes. We have cloned a complete Cherax quadricarinatus vitellogenin cDNA. The sequenced cDNA contains a 2584 aa open reading frame which shows sequence similarity to vitellogenins from other crustaceans. The mRNA encodes at least two of the previously identified vitellin components, indicating that the primary translation product is subject to post-translational modification, including proteolytic cleavage. The region close to the 3' end of the mRNA encodes a previously characterized negatively charged protein (provisionally designated p(106)). We show here that the negative charge of p(106) could be due to its ability to bind calcium. Northern blot data show that this gene is expressed as a single 8000 nt transcript and is present in the hepatopancreas of secondary-vitellogenic females. Primary vitellogenic and other tissues examined in male and female animals were negative. In sexually plastic intersex animals, removal of the androgenic gland results in vitellogenin transcription, indicating that the gene is negatively regulated by the androgenic gland. (C) 2002 Elsevier Science (USA). All rights reserved.