High throughput sequencing analysis reveals amelioration of intestinal dysbiosis by squid ink polysaccharide
High throughput sequencing analysis reveals amelioration of intestinal dysbiosis by squid ink polysaccharide
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DOI：
10.1016/j.jff.2015.11.017
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发表时间：
2016-01-01
影响因子：
5.6
通讯作者：
Tang, Qing-juan
中科院分区：
文献类型：
--
作者：
Lu, Shangyun;Zuo, Tao;Tang, Qing-juan
环磷酰胺（Cy）是临床上最常用的癌症治疗化疗药物，尽管它会损害肠道的微生物生态。这项研究的重点是利用食物来源的功能性成分来操纵肠道微生物群。采用高通量测序技术研究乌贼墨多糖（SIP）对化疗所致小鼠肠道生态失调的保护作用。在门、纲、科和属水平上的优势类群的相对丰度的变化显示了SIP的令人难以置信的影响。简而言之，SIP降低了瘤胃球菌属、嗜胆汁菌属、颤螺旋菌属、Dorea属以及尤其是粘螺菌属的丰度，其在结肠表面粘液层的早期破坏中茁壮成长并诱导炎性病症。我们的研究结果可能对使用SIP作为功能性食品成分具有重要意义，在操纵肠道微生物群方面具有潜在的治疗效用。(C)2015爱思唯尔有限公司版权所有。
Cyclophosphamide (Cy) is the most commonly used clinical chemotherapeutic drug for cancer treatment, although it damages the microbial ecology of the gut. This study focuses on the utilisation of food-derived functional components to manipulate the gut microbiota. With a mouse model injected with Cy, the protective effect of squid ink polysaccharide (SIP) on chemotherapy-induced intestinal dysbiosis was investigated by high-throughput sequencing. The shifts in relative abundance of the dominant taxa at the phylum, class, family, and genus levels show the incredible effects of SIP. In short, SIP decreases the abundance of Ruminococcus, Bilophila, Oscillospira, Dorea and, especially, Mucispirillum, which thrives in the early disruption of the colonic surface mucus layer and induces inflammatory disorders. Our results may have important implications for the use of SIP as a functional food component with potential therapeutic utility in manipulating the gut microbiota. (C) 2015 Elsevier Ltd. All rights reserved.