BACILLUS SPORULATION GENE SPOOH CODES FOR SIGMA-30 (SIGMA-H)
BACILLUS SPORULATION GENE SPOOH CODES FOR SIGMA-30 (SIGMA-H)
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DOI：
10.1128/jb.170.3.1054-1062.1988
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发表时间：
1988-03-01
影响因子：
3.2
通讯作者：
SMITH, I
中科院分区：
文献类型：
--
作者：
DUBNAU, E;WEIR, J;SMITH, I
对地衣芽孢杆菌和B.描述了枯草芽孢杆菌，并且预测的开放阅读框分别编码26，097和25，447道尔顿的蛋白质。两个spo0H基因产物彼此有91%的相同性，并且与大多数原核sigma因子有约25%的相同性。预测的蛋白质在其氨基末端具有典型的sigma因子的保守的14个氨基酸序列。针对B的spo0H基因产物产生的抗体。地衣菌特异性地与从B纯化的RNA聚合酶σ因子蛋白σ 30反应。枯草杆菌。我们认为B. licheniformis和B.用于σ 30的subtilis代码，现在称为σ H。
The DNA sequences of the spo0H genes from Bacillus licheniformis and B. subtilis are described, and the predicted open reading frames code for proteins of 26,097 and 25,447 daltons, respectively. The two spo0H gene products are 91% identical to one another and about 25% identical to most of the procaryotic sigma factors. The predicted proteins have a conserved 14-amino-acid sequence at their amino terminal end, typical of sigma factors. Antibodies raised against the spo0H gene product of B. licheniformis specifically react with RNA polymerase sigma factor protein, .sigma.30, purified from B. subtilis. We conclude that the spo0H genes of B. licheniformis and B. subtilis code for .sigma.30, now known as .sigma.H.