Theoretical investigation of improved ion trapping in matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry: independence of ion initial velocity
Theoretical investigation of improved ion trapping in matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry: independence of ion initial velocity
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DOI：
10.1016/s0168-1176(96)04542-9
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发表时间：
1997-04-01
期刊：
影响因子：
--
通讯作者：
Wanczek, KP
中科院分区：
文献类型：
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作者：
Knobeler, M;Wanczek, KP
对基质辅助激光解吸电离傅里叶变换离子回旋共振质谱（MALDI FT-ICR MS）中的开关阻滞方法进行了理论研究。MALDI产生的离子的减速与减速电位已成为实验上重要的。不幸的是，离子是根据它们的质量和初始速度来区分的。讨论了该方法的基本原理和优化方法。一个理想的，二次增加的潜力被引入到减速的所有离子的一个给定的质量，但任意的初始速度低于选定的最大值。三种不同的实现一个接近理想的电位，即一个细胞的长度减少，使用一个增加的阻滞电压，或一个细胞与校正电极。不同的解决方案的特性进行了比较与模拟。此外，它表明，与减速电位的质量上限是大于一半的正常ICR的质量上限。结果证明，引入的优化大大增加了单次激光照射后捕获离子的数量，从而提高了MALDI FT-ICR MS的灵敏度。（C）1997 Elsevier Science B. V.
A theoretical investigation of the switched retarding method is presented for matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI FT-ICR MS). Deceleration of MALDI generated ions with a retarding potential has become experimentally important. Unfortunately, the ions are discriminated with respect to their masses and initial velocities. The basics and optimization of the method are discussed. An ideal, quadratically increasing potential is introduced to decelerate all ions of a given mass but arbitary initial velocities below a chosen maximum. Three different realizations of a near ideal potential are introduced, namely a cell with a reduced length, the use of an increased retarding voltage, or a cell with correction electrodes. The characteristics of the different solutions are compared with simulations. Moreover, it is shown that the upper mass limit with the retarding potential is larger than half the upper mass limit in normal ICR. The results prove that the introduced optimization drastically increases the number of trapped ions after a single laser shot and thus the sensitivity in MALDI FT-ICR MS. (C) 1997 Elsevier Science B.V.