Expression of suppressor of cytokine signaling 1 in the peripheral blood of patients with idiopathic pulmonary fibrosis
Expression of suppressor of cytokine signaling 1 in the peripheral blood of patients with idiopathic pulmonary fibrosis
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DOI：
10.3760/cma.j.issn.0366-6999.20133038
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发表时间：
2014-06-05
影响因子：
6.1
通讯作者：
Zhou Min
中科院分区：
文献类型：
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作者：
Bao Zhiyao;Zhang Qiurui;Zhou Min
特发性肺纤维化（IPF）是一种进行性弥漫性肺实质疾病，预后差。多种细胞因子和趋化因子参与其病理生理过程。本研究旨在探讨细胞因子信号抑制因子1（SOCS-1）在IPF患者中的表达及其临床意义。采用RT-PCR方法检测受试者外周血单个核细胞（PBMC）中SOCS-1的表达。白细胞介素4（IL-4），转化生长因子β 1（TGF-β 1）和I型胶原蛋白的表达也进行了分析，在每个人使用酶联免疫吸附试验（ELISA）。描述了IPF患者的临床特征。结果与健康对照组相比，IPF患者PBMC中SOCS-1 mRNA表达明显降低，血清IL-4和TGF-β 1水平升高。SOCS-1表达水平越低，用力肺活量（FVC%）和DLCO/VA越低。结论IPF患者PBMC中存在SOCS-1 mRNA，且SOCS-1 mRNA在IPF患者中表达下调。SOCS-1的表达与IPF患者症状的严重程度相关，因此SOCS-1可能是IPF患者病情严重程度的预测因子。SOCS-1可能通过降低辅助性T细胞2型（Th 2）相关细胞因子IL-4的表达在IPF中发挥重要作用。
Background Idiopathic pulmonary fibrosis (IPF) is a progressive diffuse parenchymal disease with a poor prognosis. A variety of cytokines and chemokines are involved in its pathophysiology. The aim of this study was to evaluate the clinical features in IPF patients with the expression of suppressor of cytokine signaling 1 (SOCS-1), which acts as a negative regulator of cytokine signaling.Methods IPF patients (n=20) and healthy controls (n=16) were included in this study. The expression of SOCS-1 was analyzed in peripheral blood mononuclear cells (PBMC) of subjects using RT-PCR. Interleukin 4 (IL-4), transforming growth factor beta 1 (TGF-beta 1) and type I collagen expression were also analyzed in each individual using enzyme-linked immunosorbent assay (ELISA). The clinical characteristics of IPF patients were delineated. These results were analyzed by SPSS13.0 statistics software.Results SOCS-1 mRNA expression was significantly decreased in the PBMC of IPF patients compared with healthy controls; serum levels of IL-4 and TGF-beta 1 were higher in IPF patients. The patients with lower expression of SOCS-1 developed lower percentage of forced vital capacity (FVC%) and DLCO/VA. A patients' SOCS-1 nnRNA level was negatively correlated with serum levels of IL-4, and negatively correlated with their high-resolution computed tomography (HRCT) scores.Conclusions SOCS-1 mRNA can be detected in PBMC, and it is down-regulated in IPF patients. The expression of SOCS-1 is associated with the severity of IPF patients' symptoms, so it might be the predictor of disease severity. SOCS-1 might play an important role in IPF by reducing the expression of the T helper type 2 (Th2) cell-related cytokine IL-4.