Paraxial mesoderm contributes stromal cells to the developing kidney
Paraxial mesoderm contributes stromal cells to the developing kidney
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DOI：
10.1016/j.ydbio.2009.02.034
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发表时间：
2009-05-15
影响因子：
2.7
通讯作者：
Herzlinger, Doris
中科院分区：
文献类型：
--
作者：
Guillaume, Richard;Bressan, Michel;Herzlinger, Doris
大多数（如果不是全部的话）管状器官的发育依赖于上皮和基质祖细胞群体之间的信号传导。大多数情况下，这些谱系来自不同的胚层，这些胚层在原肠胚形成期间被指定，远在器官凝结之前。因此，器官发生的第一阶段之一是将不同的祖先群体整合成单个胚胎雏形。相比之下，控制肾发育的基质和上皮谱系都被认为来自中间中胚层，并随着肾发育而被指定。在这项研究中，我们直接分析了谱系的肾上皮细胞和间质在发育中的鸡胚使用两个独立的命运映射技术。这些实验结果证实了肾单位上皮来源于中间中胚层的假设。最重要的是，我们发现大量的肾间质起源于近轴中胚层。总的来说，这些研究表明，中胚层细分为中间和近轴域的信号可能在指定肾单位上皮细胞和肾间质谱系中发挥作用。此外，这些命运映射数据表明，肾的发展，像所有其他管状器官的发展，是依赖于从不同的胚胎组织的祖细胞整合成一个单一的雏形。(C)2009 Elsevier Inc. All rights reserved.
The development of most, if not all, tubular organs is dependent on signaling between epithelial and stromal progenitor populations. Most often, these lineages derive from different germ layers that are specified during gastrulation, well in advance of organ condensation. Thus, one of the first stages of organogenesis is the integration of distinct progenitor populations into a single embryonic rudiment. In contrast, the stromal and epithelial lineages controlling renal development are both believed to derive from the intermediate mesoderm and to be specified as the kidney develops. In this study we directly analyzed the lineage of renal epithelia and stroma in the developing chick embryo using two independent fate mapping techniques. Results of these experiments confirm the hypothesis that nephron epithelia derive from the intermediate mesoderm. Most importantly, we discovered that large populations of renal stroma originate in the paraxial mesoderm. Collectively, these studies suggest that the signals that subdivide mesoderm into intermediate and paraxial domains may play a role in specifying nephron epithelia and a renal stromal lineage. In addition, these fate mapping data indicate that renal development, like the development of all other tubular organs, is dependent on the integration of progenitors from different embryonic tissues into a single rudiment. (C) 2009 Elsevier Inc. All rights reserved.