VEGETATIVE INTERACTIONS AMONG MYCELIA OF LACCARIA BICOLOR IN PURE CULTURE AND IN SYMBIOSIS WITH PINUS BANKSIANA
VEGETATIVE INTERACTIONS AMONG MYCELIA OF LACCARIA BICOLOR IN PURE CULTURE AND IN SYMBIOSIS WITH PINUS BANKSIANA
复制标题
纯培养物和与班克斯松共生的 LACCARIA BICOLOR 菌丝体之间的营养相互作用
作者：
P. Bastide;Y. Piché;B. Kropp
研究了双色蜡伞菌株在纯培养和接种到菌根宿主后的菌丝相互作用。单核体-单核体和双核体-单核体杂交在增强核迁移的琼脂培养基上进行，以研究交配事件。第一次观察到的夹紧菌丝，它们的位置，核迁移的证据，以及发生的双核单核交配的十字架。选择1个单核-单核和2个双核-单核组合接种幼苗，比较它们与琼脂培养基上观察到的菌根相互作用。将班克松幼苗在菌丝体接种的土壤培养基中生长20周。在收获时从每个处理中选择三株幼苗，并对外生真菌reisolates进行随机扩增多态性DNA分析以鉴定基因型。该分析也在琼脂培养基上对相同杂交的菌丝体样品进行。观察到的mycobiont基因型的变化…
Mycelial interactions of Laccaria bicolor strains were studied in pure culture and after inoculation onto mycorrhizal hosts. Monokaryon–monokaryon and dikaryon–monokaryon crosses were performed on an agar medium that enhanced nuclear migration to study mating events. The first observance of clamped hyphae, their location, evidence of nuclear migration, and the occurrence of dikaryon–monokaryon matings varied among crosses. One monokaryon–monokaryon and two dikaryon–monokaryon combinations were selected for seedling inoculation to compare their mycorrhizosphere interactions with those observed on agar medium. Seedlings of Pinus banksiana were grown for 20 weeks in a mycelium inoculated soil medium. Three seedlings from each treatment were selected at harvest and ectomycorrhizae reisolates were subject to randomly amplified polymorphic DNA analysis to identify genotypes. This analysis was also done for mycelial samples of the same crosses on agar medium. Variation in the mycobiont genotype was observed for ...