Characterisation of a mural cell network in the murine pituitary gland.
Characterisation of a mural cell network in the murine pituitary gland.
复制标题
小鼠垂体壁细胞网络的表征。
DOI：
10.1111/jne.12903
复制
发表时间：
2020
影响因子：
3.2
通讯作者：
O'Hara L
中科院分区：
文献类型：
--
作者：
O'Hara L
垂体的前叶和中叶由内分泌细胞、血管系统和支持细胞（如滤泡星状细胞）组成。滤泡星状细胞形成一个网络，在垂体中具有几种假定的作用，包括产生旁分泌信号分子和细胞因子，协调内分泌细胞激素释放，吞噬作用和结构支持。大鼠滤泡星状细胞的特征是表达S100 B蛋白，而人类滤泡星状细胞的特征是表达胶质细胞酸性蛋白。然而，有证据表明在垂体前叶中存在另一种支持细胞网络，其具有壁细胞的性质，例如血管平滑肌细胞和周细胞。本研究旨在确定表达壁细胞标志物血小板衍生生长因子受体β（PDGFRβ）的细胞在小鼠垂体中的分布，并确定这些细胞是否为滤泡星状细胞。通过免疫组织化学定位，我们确定小鼠垂体中约80%的PDGFRβ+细胞具有非血管周围位置，20%为周细胞。对PDGFRβ+细胞的磁性细胞分选群体中的基因表达进行的研究表明，尽管大多数位于非血管周围，但该群体富集了壁细胞标志物，但未富集大鼠或人卵泡星状细胞标志物。这是通过免疫组织化学证实的。本研究得出结论，小鼠垂体前叶中存在壁细胞网络，并且该群体不表达充分表征的人或大鼠滤泡星状细胞标志物。
The anterior and intermediate lobes of the pituitary are composed of endocrine cells, as well as vasculature and supporting cells, such as folliculostellate cells. Folliculostellate cells form a network with several postulated roles in the pituitary, including production of paracrine signalling molecules and cytokines, coordination of endocrine cell hormone release, phagocytosis, and structural support. Folliculostellate cells in rats are characterised by expression of S100B protein, and in humans by glial fibrillary acid protein. However, there is evidence for another network of supporting cells in the anterior pituitary that has properties of mural cells, such as vascular smooth muscle cells and pericytes. The present study aims to characterise the distribution of cells that express the mural cell marker platelet derived growth factor receptor beta (PDGFRβ) in the mouse pituitary and establish whether these cells are folliculostellate. By immunohistochemical localisation, we determine that approximately 80% of PDGFRβ+ cells in the mouse pituitary have a non‐perivascular location and 20% are pericytes. Investigation of gene expression in a magnetic cell sorted population of PDGFRβ+ cells shows that, despite a mostly non‐perivascular location, this population is enriched for mural cell markers but not enriched for rat or human folliculostellate cell markers. This is confirmed by immunohistochemistry. The present study concludes that a mural cell network is present throughout the anterior pituitary of the mouse and that this population does not express well‐characterised human or rat folliculostellate cell markers.