Immunocytochemical localization of glutamate in normal and detached cat retina.
Immunocytochemical localization of glutamate in normal and detached cat retina.
复制标题
谷氨酸在正常和脱离猫视网膜中的免疫细胞化学定位。
DOI：
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发表时间：
1998
影响因子：
4.4
通讯作者：
Y. Uji
中科院分区：
文献类型：
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作者：
M. Sasoh;Ning Ma;S. Yoshida;Reiji Semba;Y. Uji
目的
谷氨酸在哺乳动物视网膜的免疫反应性已普遍观察到使用浸泡固定。作者研究了脱离的猫视网膜中的谷氨酸免疫反应性，并通过灌注快速固定重新评估了正常视网膜中的这种活性。
方法
用玻璃微吸管将0.25%透明质酸钠注入猫视网膜下腔，造成单侧视网膜脱离。分离后10 min和60 min，用1%戊二醛和4%甲醛混合液灌注固定，然后进行常规光镜和电镜免疫细胞化学检查。
结果
与以前的报道相比，浸泡固定，在正常的视网膜内段不谷氨酸免疫阳性。视网膜脱离后10分钟和60分钟内节显示强烈的谷氨酸免疫反应。
结论
光感受器内节谷氨酸免疫反应可能是强烈缺血引起的死后变化。灌注固定是至关重要的，当研究谷氨酸在视网膜中的免疫细胞化学分布。
PURPOSE
Glutamate immunoreactivity in the mammalian retina has generally been observed using immersion fixation. The authors investigated glutamate immunoreactivity in the detached cat retina and reevaluated this activity in the normal retina using rapid fixation by perfusion.
METHODS
Unilateral retinal detachment was produced in cats by injecting 0.25% sodium hyaluronate into the subretinal space using a glass micropipet. The eyes were fixed by perfusion with a mixture of 1% glutaraldehyde and 4% formaldehyde 10 minutes and 60 minutes after detachment, and then they were examined by conventional light and electron microscopic immunocytochemistry.
RESULTS
In contrast to previous reports based on immersion fixation, the inner segment was not glutamate immunopositive in the normal retina. The inner segment showed intense glutamate immunoreactivity 10 minutes and 60 minutes after retinal detachment.
CONCLUSIONS
Glutamate immunoreactivity in photoreceptor inner segments may be a postmortem change induced by strong ischemia. Perfusion fixation is of critical importance when studying the immunocytochemical distribution of glutamate in the retina.
影响因子：
4.4
作者：
S. Geller;G. Lewis;Don H. Anderson;S. Fisher
通讯作者：
S. Geller;G. Lewis;Don H. Anderson;S. Fisher