DEFORMATION AND FLOW OF RED-BLOOD-CELLS IN A SYNTHETIC LATTICE - EVIDENCE FOR AN ACTIVE CYTOSKELETON
DEFORMATION AND FLOW OF RED-BLOOD-CELLS IN A SYNTHETIC LATTICE - EVIDENCE FOR AN ACTIVE CYTOSKELETON
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DOI：
10.1016/s0006-3495(95)80443-1
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发表时间：
1995-06-01
影响因子：
3.4
通讯作者：
BITENSKY, M
中科院分区：
文献类型：
--
作者：
BRODY, JP;HAN, YQ;BITENSKY, M
我们介绍了使用微加工技术在硅晶片上构建一个2.5至4微米（mu）宽的通道的合成毛细管床。建立一个流体压力梯度，使我们能够同时使用光学显微镜观察数百个细胞以生理速度流过床。我们发现一个大的红细胞之间的流动性分布流过的结构，较小的通道提供了一个更大的阻抗比较大的流动，表明动力阻力的变化提供了起源的分布。特定细胞的移动性与细胞直径不相关，但似乎与细胞的细胞内钙浓度负相关，如通过钙结合染料fluo-3 AM的荧光测定的。此外，我们能够使用我们的阵列的并行处理性质来观察孤立的事件，其中红细胞的刚性似乎在几个数量级上突然变化，因为它阻塞了阵列中的通道。
We introduce the use of microfabrication techniques to construct on a silicon wafer a synthetic capillary bed with 2.5- to 4-micron (mu)-wide channels. Establishment of a fluid pressure gradient allowed us to observe simultaneously using optical microscopy hundreds of cells flowing through the bed at physiological speeds. We find a large distribution of mobilities among red cells flowing through the structure; smaller channels provide a greater impedance to flow than larger ones, indicating that kinetic drag variations provide the origin of the distribution. The mobility of a particular cell is not correlated with the cell diameter but appears to be inversely correlated with intracellular calcium concentration of the cell, as determined by fluorescence of the calcium-binding dye fluo-3 AM. Also, we are able to use the parallel processing nature of our arrays to observe isolated events where the rigidity of the red cell seems to change suddenly over several orders of magnitude as it blocks a channel in the array.