Influence of the site of injury on the spontaneous healing response in a rat model of total rupture of the anterior cruciate ligament
Influence of the site of injury on the spontaneous healing response in a rat model of total rupture of the anterior cruciate ligament
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DOI：
10.1080/03008207.2021.1889529
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发表时间：
2021-03-06
影响因子：
2.9
通讯作者：
Kanemura, Naohiko
中科院分区：
文献类型：
--
作者：
Kano, Takuma;Kokubun, Takanori;Kanemura, Naohiko
目的：前交叉韧带（ACL）损伤的愈合能力非常差;然而，最近已显示通过保守治疗可以自行愈合。在这项研究中，我们评估了损伤部位对ACL完全横向撕裂后愈合过程的影响，采用大鼠模型。材料和方法：将58只成年Wistar大鼠随机分为ACL损伤组：受控异常运动-中部（CAM-MP），受控异常运动-股骨侧（CAM-FS），ACL横断-中部（ACLT-MP），或ACL横断-股骨侧（ACLT-FS）损伤组。ACLT-MP和CAM-MP组ACL中部完全横断，ACLT-FS和CAM-FS组ACL股骨侧完全横断。两个CAM组均接受关节外制动，以控制异常胫骨平移。动物被允许全笼活动，直到牺牲postoperative的组织学和生物力学assessment.Results：被发现在残余韧带长度的比例之间的CAM-MP和CAM-FS组的显着差异，证明了每个模型的有效性。在CAM-MP和CAM-FS组中观察到损伤的ACL的自发愈合，而在ACLT-MP和ACLT-FS组中未观察到。CAM-MP组和CAM-FS组在损伤后8周的力学强度无明显差异，但在损伤后12周，前者的力学强度优于后者。结论：ACL中段和股骨侧损伤可采用保守治疗，以促进其自然愈合。
Aim: The healing ability of the anterior cruciate ligament (ACL) injury is very poor; however, it has recently been shown to undergo self-healing with conservative treatments. In this study, we evaluated the influence of the site of injury on the healing process after complete transverse tear of ACL using a rat model.Materials and Methods: A total of 58 skeletally mature Wistar rats were randomly assigned to various ACL injury groups: controlled abnormal movement-mid-portion (CAM-MP), controlled abnormal movement-femoral side (CAM-FS), ACL transection-mid-portion (ACLT-MP), or ACL transection-femoral side (ACLT-FS) injury groups. The ACL was completely transected in the mid-portion in the ACLT-MP and CAM-MP groups, and on the femoral side in the ACLT-FS and CAM-FS groups. Both CAM groups underwent extra-articular braking to control for abnormal tibial translation. The animals were allowed full cage activity until sacrifice postoperatively for histological and biomechanical assessment.Results: Significant differences were found in the ratios of residual ligament lengths between the CAM-MP and CAM-FS groups, demonstrating the validity of each model. Spontaneous healing of the injured ACL was observed in the CAM-MP and CAM-FS groups but not in the ACLT-MP and ACLT-FS groups. The mechanical strength of the healing ACL did not differ between the CAM-MP and CAM-FS groups 8 weeks after injury; however, the former had better mechanical strength than the latter 12 weeks after the injury.Conclusion: ACL injuries in the mid-portion and on the femoral side may be treated with conservative therapy for spontaneous healing.