INFLUENCE OF DIET ON DISTRIBUTION OF CARBON ISOTOPES IN ANIMALS
INFLUENCE OF DIET ON DISTRIBUTION OF CARBON ISOTOPES IN ANIMALS
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DOI：
10.1016/0016-7037(78)90199-0
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发表时间：
1978-01-01
影响因子：
5
通讯作者：
EPSTEIN, S
中科院分区：
文献类型：
--
作者：
DENIRO, MJ;EPSTEIN, S
研究了食物对动物[卤虫（卤虫）、秀丽隐杆线虫（线虫）、拟丽蝇（丽蝇）、死索虫（蛾）、蜗牛（蜗牛）、黑足蝗（蚱蜢）、小鼠（小鼠）]体内碳同位素分布的影响。（小鼠）、家蝇（家蝇）、细条长蝽（Oncopeltus fasciatus）（乳草蝽）和Sitophilus spp.通过分析在实验室中以恒定C同位素组成的饮食生长的动物来研究[（象鼻虫）]。动物全身的同位素组成反映了其饮食的同位素组成，但该动物平均富集δ 13 C约1.permill。相对于饮食。在4例检查中的3例中，整个身体相对于饮食的13 C富集通过呼吸CO2的13 C消耗来平衡。动物的整个身体和它们的食物之间的同位素关系对于以相同食物饲养的不同物种和以不同食物饲养的相同物种是相似的。以相同饮食饲养的物种的个体的全身的δ 13 C值可以相差高达2 ‰。组织的13 C/12 C比率与饮食的13 C/12 C比率之间的关系取决于组织的类型和饮食的性质。许多同位素之间的关系，主要的生化组分，即脂质，碳水化合物和蛋白质组分，定性保存作为饲料碳纳入动物。动物和其饮食中的生物化学级分的δ 13 C值之间的差异可以大至3 ‰。生物化学成分胶原蛋白、几丁质和贝壳的不溶性有机部分的δ 13 C值与饮食的同位素组成有关，所有这些成分通常保存在化石材料中。这些结果表明，有可能根据动物C的13 C/12 C比值的测定进行膳食分析。在大多数情况下，对动物总碳的分析将比对单个组织、生化组分或生化成分的分析提供更好的饮食测量。该方法的精确度的限制通常将其应用限制于这样的情况，其中饮食来源于δ 13 C值具有相对较大差异的来源，例如陆生生物与水生生物或C3植物与C4植物。该方法应适用于化石和生物材料。
The influence of diet on the distribution of C isotopes in animals [Artemia salina (brine shrimp), Caenorhabditis elegans (nematode), Calliphora vicina (blowfly), Desmia funeralis (moth), Helix aspersa (snail), Melanoplus sanguinipes (grasshopper), Mus spp. (mice), Musca domestica (house fly), Oncopeltus fasciatus (milkweed bug) and Sitophilus spp. (weevils)] was investigated by analyzing animals grown in the laboratory on diets of constant C isotopic composition. The isotopic composition of the whole body of an animal reflects the isotopic composition of its diet, but the animal is on average enriched in .delta.13C by about 1.permill. relative to the diet. In 3 of 4 cases examined, the 13C enrichment of the whole body relative to the diet is balanced by a 13C depletion of the respired CO2. The isotopic relationships between the whole bodies of animals and their diets are similar for different species raised on the same diet and for the same species raised on different diets. The .delta.13C values of whole bodies of individuals of a species raised on the same diet may differ by up to 2.permill.. The relationship between the 13C/12C ratio of a tissue and the 13C/12C ratio of the diet depends both on the type of tissue and on the nature of the diet. Many of the isotopic relationships among the major biochemical fractions, namely the lipid, carbohydrate and protein fractions, are qualitatively preserved as diet carbon is incorporated into the animal. The difference between the .delta.13C values of a biochemical fraction in an animal and in its diet may be as large as 3.permill.. The .delta.13C values of the biochemical components collagen, chitin and the insoluble organic fraction of shells, all of which are often preserved in fossil material, are related to the isotopic composition of the diet. These results indicate that it will be possible to perform dietary analysis based on the determination of the 13C/12C ratio of animal C. Analysis of the total animal carbon will in most cases provide a better measure of diet than the analysis of individual tissues, biochemical fractions, or biochemical components. The limits of accuracy of this method will generally restrict its application to situations in which the diet is derived from sources with relatively large differences in their .delta.13C values, such as terrestrial vs. aquatic organisms or C3 vs. C4 plants. The method should be applicable to fossil and living material.