Simultaneous Determination of Eight Quinolone Antibacterials in Meat and Fish by High Performance Liquid Chromatography
Simultaneous Determination of Eight Quinolone Antibacterials in Meat and Fish by High Performance Liquid Chromatography
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高效液相色谱法同时测定肉类和鱼类中的八种喹诺酮类抗菌药物
DOI：
10.3358/shokueishi.36.62
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
H. Nakazawa
中科院分区：
文献类型：
--
作者：
M. Horie;Koichi Saito;N. Nose;H. Nakazawa
建立了一种简便、快速、可靠的高效液相色谱法同时测定肉、鱼中8种喹诺酮类药物（恶喹酸、萘地酸、氟甲喹、吡罗米地酸、苯氧氟沙星、达氟沙星、恩诺沙星和氧氟沙星）残留量的方法。用0.2%偏磷酸-乙腈（7：3）提取药物，然后进行Bond Elut C18净化程序。采用Wakosil II 5C 18-HG色谱柱（150×4.6mm i. d.）以0.05M磷酸盐缓冲液（pH2.5）-乙腈（65：35）（含3.5mM十二烷基硫酸钠（SDS））为移动的相，流速0.5ml/min。达氟沙星在0.1 ~ 2ng范围内线性关系良好，其它药物在1 ~ 20 ng范围内线性关系良好。在0.2μg/g添加水平下，回收率为80.8- 92.1%，精密度高。各药物的检出限为0.01μg/g。将该方法应用于残留恶喹酸的热稳定性研究。恶喹酸具有良好的热稳定性。
A simple, rapid and reliable high performance liquid chromatographic (HPLC) method for the simultaneous determination of eight quinolones (oxolinic acid, nalidixic acid, flumequine, piromidic acid, benofloxacin, danofloxacin, enrofloxacin and ofloxacin) in meat and fish has been developed. The drugs were extracted with 0.2% metaphosphoric acid-acetonitrile (7:3), followed by a Bond Elut C18 clean-up procedure. The HPLC separation was carried out on a Wakosil II 5C18-HG column (150×4.6mm i. d.) with 0.05M phosphate buffer (pH 2.5)-ac-etonitrile (65:35) containing 3.5mM sodium dodecyl sulfate (SDS) as the mobile phase at a flow rate of 0.5ml/min. The drugs were detected by UV detection (at 282nm) and fluorescence detection (wavelength programing). The calibration graphs were rectilinear from 0.1 to 2ng for danofloxacin, and from 1 to 20ng for other drugs. The recoveries of the drugs from meat and fish fortified at the level of 0.2μg/g were 80.8-92.1%, with high precision. The limit of detection was 0.01μg/g for each drug. The method was applied in thermal stability studies of residual oxolinic acid. The thermal stability of oxolinic acid was excellent.