Protocol to establish a lung adenocarcinoma immunotherapy allograft mouse model with FACS and immunofluorescence-based analysis of tumor response
Protocol to establish a lung adenocarcinoma immunotherapy allograft mouse model with FACS and immunofluorescence-based analysis of tumor response
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DOI：
10.1016/j.xpro.2021.100595
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Sun Shuguo
中科院分区：
文献类型：
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作者：
Zhengxin Peng;Man Yu;Lin Jiaming;Dong Tianqi;Zhang Xiao;Shi Mingjun;Qin Min;Li Shasha;Guo Wencong;Zhang Huixia;Sun Shuguo
Anti-PD-1/PD-L1 therapy shows long-term effects in many cancer types, but resistance and relapse remain the main limitations of this therapy. Here, we describe a protocol to evaluate the tumor response to immunotherapy in a mouse lung cancer model. The protocol includes the establishment of the lung cancer mouse model, anti-PD-1 treatment, tumor-infiltrating lymphocyte isolation, immunofluorescence, and flow cytometry analysis. This protocol can also be applied to other cancer types and immunotherapies. For complete details on the use and execution of this protocol, please refer to Yu et al. (2021)
