ACID-BASE BEHAVIOR OF SEPARATED CANINE RENAL TUBULE CELLS
ACID-BASE BEHAVIOR OF SEPARATED CANINE RENAL TUBULE CELLS
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DOI：
10.1152/ajplegacy.1968.214.5.1155
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发表时间：
1968-01-01
影响因子：
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通讯作者：
RELMAN, AS
中科院分区：
文献类型：
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作者：
STRUYVEN.A;MORRISON, RB;RELMAN, AS
在分离的犬肾小管中，使用菊粉作为非细胞水的测量值，根据DMO [5，5-二甲基-2，4-恶唑烷二酮] 14 C的分布计算细胞内pH。在正常的P（X）>2 [二氧化碳压力]和细胞外[HCO 3-]下，发现肾细胞更碱性（平均pH 7. 32)比其他大多数哺乳动物细胞都多。细胞酸度变化的直接线性关系，细胞外酸度时，后者被改变的系统变化的PCO 2或细胞外[HCO 3-]，但PCO 2有一个显着更大的影响。通过添加10-5 M乙酰唑胺抑制碳酸酐酶显着增加细胞的pH值。无论是急性K耗竭，也不快速补充显着影响细胞的pH值。结果表明，肾小管细胞是相对碱性的，因为它们分泌酸的分泌过程似乎只是间接连接到碳酸酐酶。[long破折号]作者。
In separated canine renal tubules intra-cellular pH was calculated from the distribution of DMO [5, 5-dimethyl-2, 4-oxazolidinedione] 14C, using inulin as a measurement of noncellular water. At normal P(X>2 [carbon dioxide pressure] and extracellular [HCO3-] renal cells were found to be more alkaline (mean pH 7. 32) than most other mammalian cells. Cell acidity varied in direct linear relation to extracellular acidity when the latter was changed by systematic variation of either PCO2 or extracellular [HCO3-], but PCO2 had a significantly greater effect. Inhibition of carbonic anhydrase by addition of 10-5 M acetazolamide significantly increased cellular pH. Neither acute K depletion nor rapid repletion significantly affected cell pH. The results suggest that renal tubular cells are relatively alkaline because they secrete acid; the secretory process appears to be only indirectly linked to carbonic anhydrase. [long dash]Authors.