In vitro fermentation of alginate and its derivatives by human gut microbiota
In vitro fermentation of alginate and its derivatives by human gut microbiota
复制标题
人体肠道菌群体外发酵海藻酸盐及其衍生物
DOI：
10.1016/j.anaerobe.2016.02.003
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发表时间：
2016-06-01
期刊：
影响因子：
2.3
通讯作者：
Wang, Xin
中科院分区：
文献类型：
--
作者：
Li, Miaomiao;Li, Guangsheng;Wang, Xin
海藻酸盐（Alg）在东亚作为食品配料有着悠久的历史。然而，负责降解藻酸盐及其衍生物的人类肠道微生物尚未完全了解。在这里，我们报告，藻酸盐和低分子聚合物衍生物的甘露糖醛酸低聚糖（MO）和古洛糖醛酸低聚糖（GO）可以完全降解，并利用在不同的速度从6个中国人的粪便微生物群。而藻酸丙二醇酯硫酸钠（PSS）的衍生物不水解。从人粪便样品中分离出具有显著降解Alg、MO和GO能力的细菌，并鉴定为卵形拟杆菌、溶木聚糖拟杆菌和多形拟杆菌。Alg、MO和GO均能提高短链脂肪酸（SCFA）的产量，但GO的SCFA产量最高。结果表明，海藻酸盐及其衍生物在人体肠道内可被特定细菌降解，为海藻酸盐及其衍生物作为特殊食品添加剂对人体健康的影响提供了依据。(C)2016爱思唯尔有限公司版权所有
Alginate (Alg) has a long history as a food ingredient in East Asia. However, the human gut microbes responsible for the degradation of alginate and its derivatives have not been fully understood yet. Here, we report that alginate and the low molecular polymer derivatives of mannuronic acid oligosaccharides (MO) and guluronic acid oligosaccharides (GO) can be completely degraded and utilized at various rates by fecal microbiota obtained from six Chinese individuals. However, the derivative of propylene glycol alginate sodium sulfate (PSS) was not hydrolyzed. The bacteria having a pronounced ability to degrade Alg, MO and GO were isolated from human fecal samples and were identified as Bacteroides ovatus, Bacteroides xylanisolvens, and Bacteroides thetaiotaomicron. Alg, MO and GO can increase the production level of short chain fatty acids (SCFA), but GO generates the highest level of SCFA. Our data suggest that alginate and its derivatives could be degraded by specific bacteria in the human gut, providing the basis for the impacts of alginate and its derivates as special food additives on human health. (C) 2016 Elsevier Ltd. All rights reserved.