Dissection of cis-regulatory elements in the C. elegans Hox gene egl-5 promoter.
Dissection of cis-regulatory elements in the C. elegans Hox gene egl-5 promoter.
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线虫 Hox 基因 egl-5 启动子中顺式调控元件的剖析。
DOI：
10.1016/j.ydbio.2004.09.012
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Emmons,ScottW
中科院分区：
文献类型：
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作者：
Teng,Yingqi;Girard,Lisa;Ferreira,HenriqueB;Sternberg,PaulW;Emmons,ScottW
Hox基因是高度保守的片段身份基因，以其复杂的表达模式和不同的靶点而闻名。在这里，我们分析了秀丽隐杆线虫Hox基因egl-5中的顺式调控元件，该基因在线虫后部的多个组织中表达。我们利用系统发育足迹来有效地识别顺式调控元件，并通过gfp报告器和组织特异性拯救实验对这些元件进行了表征。我们发现，egl-5的复杂表达模式是多个组织或局部区域特异性激活子序列活动的累积结果，这些激活子序列在相关的桥状隐杆线虫中在序列和近乎完美的顺序上都是保守的。详细分析了两个保守的调控块包含多个积极和消极作用因子的位点。其中一个区域可能通过Wnt途径促进某些细胞中egl-5的激活。在不适当的组织中，正作用区域被作用于启动子内其他位点的额外负作用通路所抑制。我们的分析使我们能够暗示几个新的调控因子对控制egl-5表达具有重要意义。
Hox genes are highly conserved segmental identity genes well known for their complex expression patterns and divergent targets. Here we present an analysis of cis-regulatory elements in the Caenorhabditis elegans Hox gene egl-5, which is expressed in multiple tissues in the posterior region of the nematode. We have utilized phylogenetic footprinting to efficiently identify cis-regulatory elements and have characterized these with gfp reporters and tissue-specific rescue experiments. We have found that the complex expression pattern of egl-5 is the cumulative result of the activities of multiple tissue or local region-specific activator sequences that are conserved both in sequence and near-perfect order in the related nematode Caenorhabditis briggsae. Two conserved regulatory blocks analyzed in detail contain multiple sites for both positively and negatively acting factors. One of these regions may promote activation of egl-5 in certain cells via the Wnt pathway. Positively acting regions are repressed in inappropriate tissues by additional negative pathways acting at other sites within the promoter. Our analysis has allowed us to implicate several new regulatory factors significant to the control of egl-5 expression.