Anisotropically oriented electrospun matrices with an imprinted periodic micropattern: a new scaffold for engineered muscle constructs
Anisotropically oriented electrospun matrices with an imprinted periodic micropattern: a new scaffold for engineered muscle constructs
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DOI：
10.1088/1748-6041/8/2/021001
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发表时间：
2013-04-01
影响因子：
4
通讯作者：
Giraud, Marie-Noelle
中科院分区：
文献类型：
--
作者：
Guex, Anne Geraldine;Birrer, Dominique Lisa;Giraud, Marie-Noelle
工程化肌肉构建体为不可逆损伤的骨骼肌的再生或替代提供了一个有前途的前景。然而，天然肌肉组织的高度有序结构在支架开发过程中需要特别考虑。先前已经采用了多种方法来设计具有凹槽微图案或平行排列纤维的各向异性结构化基底。在这项研究中，我们报告的指导作用的支架，结合这两种方法，定向纤维和沟槽地形。通过静电纺丝到一个拓扑结构的收集器，矩阵的平行取向的聚（ε-己内酯）纤维与90 μ m周期性的压印波形拓扑。无微图案的随机取向纤维或平行取向纤维的基质作为对照。如前所示，未图案化的平行取向的基板诱导肌管取向，其平行于纤维方向。有趣的是，图案添加诱导的肌管的取向在24度（统计中位数）的角度相对于纤维取向。与没有图案的对齐基底相比，对齐的微图案基底上的肌管长度显著增加（436 +/-245 μ m对365 +/-212 μ m; p < 0.05）。我们报告了一种创新的，但简单的设计，以产生微图案的静电纺丝支架，诱导一个意想不到的肌管方向和肌管长度的增加。
Engineered muscle constructs provide a promising perspective on the regeneration or substitution of irreversibly damaged skeletal muscle. However, the highly ordered structure of native muscle tissue necessitates special consideration during scaffold development. Multiple approaches to the design of anisotropically structured substrates with grooved micropatterns or parallel-aligned fibres have previously been undertaken. In this study we report the guidance effect of a scaffold that combines both approaches, oriented fibres and a grooved topography. By electrospinning onto a topographically structured collector, matrices of parallel-oriented poly(e-caprolactone) fibres with an imprinted wavy topography of 90 mu m periodicity were produced. Matrices of randomly oriented fibres or parallel-oriented fibres without micropatterns served as controls. As previously shown, un-patterned, parallel-oriented substrates induced myotube orientation that is parallel to fibre direction. Interestingly, pattern addition induced an orientation of myotubes at an angle of 24 degrees (statistical median) relative to fibre orientation. Myotube length was significantly increased on aligned micropatterned substrates in comparison to that on aligned substrates without pattern (436 +/- 245 mu m versus 365 +/- 212 mu m; p < 0.05). We report an innovative, yet simple, design to produce micropatterned electrospun scaffolds that induce an unexpected myotube orientation and an increase in myotube length.