Exogenous proline and glycinebetaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells
Exogenous proline and glycinebetaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells
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DOI：
10.1016/j.jplph.2009.04.002
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Murata, Yoshiyuki
中科院分区：
文献类型：
--
作者：
Islam, Mohammad Muzahidul;Hoque, Anamul;Murata, Yoshiyuki
环境胁迫，包括重金属胁迫，会对植物造成氧化损伤。脯氨酸和甜菜碱诱导的植物抗氧化防御系统的上调可以减轻氧化胁迫对植物的伤害。研究了外源脯氨酸和甜菜碱对镉（Cd）胁迫下烟草嫩黄2号（BY-2）细胞生长、脯氨酸和甜菜碱积累、膜脂过氧化和抗氧化酶活性的保护作用。镉胁迫（在100 μ M镉）引起的BY-2细胞的生长的显着抑制，脯氨酸和甜菜碱显着减轻这种抑制。此外，脯氨酸的缓解效果比甜菜碱更明显。镉胁迫导致培养细胞中镉和内源脯氨酸的积累，脂质过氧化和过氧化物酶（POX）活性增加，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性降低。外源脯氨酸处理降低了Cd胁迫下膜脂过氧化作用，提高了SOD和CAT活性，但没有降低Cd含量;外源甜菜碱处理降低了膜脂过氧化作用，提高了CAT活性，但降低了Cd积累量。此外，外源脯氨酸和甜菜碱分别促进了镉胁迫下BY-2细胞脯氨酸和甜菜碱的积累。本研究表明，脯氨酸和甜菜碱通过不同的机制赋予烟草BY-2细胞对镉胁迫的耐受性。(C)2009年Elsevier GrnbH。All rights reserved.
Environmental stress, including heavy metal stress, can cause oxidative damage to plants. Up-regulation of the antioxidant defense system induced by proline and glycinebetaine (betaine) alleviates the damaging effects of oxidative stress in plants. Here, we investigated the protective effects of exogenously applied proline and betaine on growth, accumulation of proline and betaine, lipid peroxidation and activity of antioxidant enzymes in cultured tobacco Bright Yellow-2 (BY-2) cells exposed to cadmium (Cd) stress. Cadmium stress (at 100 mu M Cd) caused a significant inhibition of the growth of BY-2 cells, and both proline and betaine significantly mitigated this inhibition. In addition, the mitigating effect of proline was more pronounced than that of betaine. Cadmium stress leads to an accumulation of Cd and endogenous proline in cultured cells, increased lipid peroxidation and peroxidase (POX) activity, and decreased activity of superoxide dismutase (SOD) and catalase (CAT). Exogenous application of proline resulted in a decrease in lipid peroxidation and an increase in SOD and CAT activities without reducing Cd contents under Cd stress, while application of betaine resulted in a decrease in lipid peroxidation and an increase in CAT activity with reducing Cd accumulation. Furthermore, exogenous proline and betaine intensified the accumulation of proline and betaine in Cd-stressed BY-2 cells, respectively. The present study suggests that proline and betaine confer tolerance to Cd stress in tobacco BY-2 cells by different mechanisms. (C) 2009 Elsevier GrnbH. All rights reserved.