Transfection types, methods and strategies: a technical review.
Transfection types, methods and strategies: a technical review.
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作者：
Chong ZX;Yeap SK;Ho WY
转染是将外源核酸插入真核细胞的一种现代而有效的方法。由于能够改变宿主细胞的遗传内容，因此该过程在研究正常细胞过程、疾病分子机制和基因治疗效果方面具有广泛的应用。在这篇综述中，我们总结和比较了各种文献报道的各种转染方法的特点，优势和局限性，转染核酸的类型，转染控制和方法来评估转染效率的结果。随着大量的选择可用的方法，我们希望这篇评论将有助于研究人员，特别是那些新的领域，在他们的决定，适当的转染协议或策略，为他们的实验目标。
Transfection is a modern and powerful method used to insert foreign nucleic acids into eukaryotic cells. The ability to modify host cells’ genetic content enables the broad application of this process in studying normal cellular processes, disease molecular mechanism and gene therapeutic effect. In this review, we summarized and compared the findings from various reported literature on the characteristics, strengths, and limitations of various transfection methods, type of transfected nucleic acids, transfection controls and approaches to assess transfection efficiency. With the vast choices of approaches available, we hope that this review will help researchers, especially those new to the field, in their decision making over the transfection protocol or strategy appropriate for their experimental aims.
影响因子：
11
作者：
Tsen SW;Wu CY;Meneshian A;Pai SI;Hung CF;Wu TC
通讯作者：
Wu TC
DOI：
10.1007/978-1-62703-311-4_5
发表时间：
2013
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Liang, Wanling;Mason, A James;Lam, Jenny K W
通讯作者：
Lam, Jenny K W