A gene expression map of the Arabidopsis root
A gene expression map of the Arabidopsis root
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DOI：
10.1126/science.1090022
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发表时间：
2003-12-12
期刊：
影响因子：
56.9
通讯作者：
Benfey, PN
中科院分区：
文献类型：
--
作者：
Birnbaum, K;Shasha, DE;Benfey, PN
器官内基因表达的全局图可以识别在局部域中具有协调表达的基因，从而将基因活性与细胞命运和组织特化相关联。在这里，我们提出了超过22，000个基因在拟南芥根中的表达定位。基因表达被映射到15个不同的区域的根，对应于细胞类型和组织在进行性发育阶段。基因表达的模式跨越传统的解剖学界限，并显示激素反应的盒。染色体聚类确定了一些共调节基因。这个表达图谱将基因组与特定的细胞命运相关联，并应用于指导反向遗传学。
A global map of gene expression within an organ can identify genes with coordinated expression in localized domains, thereby relating gene activity to cell fate and tissue specialization. Here, we present localization of expression of more than 22,000 genes in the Arabidopsis root. Gene expression was mapped to 15 different zones of the root that correspond to cell types and tissues at progressive developmental stages. Patterns of gene expression traverse traditional anatomical boundaries and show cassettes of hormonal response. Chromosomal clustering defined some coregulated genes. This expression map correlates groups of genes to specific cell fates and should serve to guide reverse genetics.