Sex ratio of Plasmodium falciparum gametocytes in inhabitants of Dielmo, Senegal
Sex ratio of Plasmodium falciparum gametocytes in inhabitants of Dielmo, Senegal
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DOI：
10.1017/s0031182003003299
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发表时间：
2003-07-01
期刊：
影响因子：
2.4
通讯作者：
Trape, JF
中科院分区：
文献类型：
--
作者：
Robert, V;Sokhna, CS;Trape, JF
在塞内加尔一个完全流行的村庄Dielmo进行了为期4个月的疟疾密集传播流行病学调查。每周为每个居民采集两份厚血涂片。对50例恶性疟原虫配子体（配子前体）的性比进行了研究。所有年龄组都有代表（平均19.7岁;范围：2个月至75岁）;其中42人（84%）没有接受抗疟治疗。总共检查了668个厚涂片，直至计数到100个配子体或40分钟。共观察到11204个配子体，平均性别比为0.346（95% CI 0.317-0.374），即2.89个雌性/1个雄性。在至少观察到10个配子体的284个厚涂片中，雄性配子体的平均百分比为27.8%，范围为0- 82%。在配子体载体之间以及来自同一配子体载体的厚涂片之间观察到很大的变异性。进行了多变量分析，强调只有2个变量对性别比有显著影响。贫血与男性比例增加相关（如果红细胞压积率< 32%，则男性配子体的患病率比[PPR]乘以1.65），配子体波与女性配子体比例增加相关（在配子体血症高峰期间和该高峰后2周，PRR乘以0.48）。对性别比无显著影响的变量有：年龄、性别、配子体携带者的临床状态和镰状细胞性状状态、无性寄生虫密度、奎宁治疗和配子体密度（考虑其波动时）。这些结果进行了讨论方面可能的差异生产，死亡率或隔离的一个配子体性别和选择性优势的传播寄生虫。
An epidemiological survey was conducted during a 4-month period of intense malaria transmission in Dielmo, a holoendemic Senegalese village. Two thick blood smears per inhabitant were collected weekly. The sex ratio of Plasmodium falciparum gametocytes (gamete precursors) was studied in 50 gametocyte carriers. All age classes were represented (mean 19.7 years; range: 2 months - 75 years); 42 (84%) of them did not receive antimalarial treatment. Overall 668 thick smears were examined until 100 gametocytes had been counted or for 40 min. A total of 11204 gametocytes were observed with a mean sex ratio of 0.346 (95% CI 0.317-0.374), i.e. 2.89 females per 1 male. Among the 284 thick smears in which at least 10 gametocytes were observed, the mean percentage of male gametocytes was 27.8%, with a range of 0-82%. Great variability was observed between gametocyte carriers and also between thick smears from the same gametocyte carrier. A multivariate analysis was performed which highlighted the fact that only 2 variables had a significant effect on the sex ratio. Anaemia was associated with an increased percentage of males (Prevalence Rate Ratio [PPR] of male gametocytes was multiplied by 1.65 if haematocrit rate < 32%) and a wave of gametocytes was associated with an increased percentage of female gametocytes (PRR was multiplied by 0.48 during the peak of gametocytaemia and for the 2 weeks following this peak). The variables without significant effect on sex ratio were: age, sex, clinical status and sickle cell trait status of the gametocyte carrier, density of asexual parasites, quinine treatment, and gametocyte density (when taking account of its waves). These results are discussed in regard of possible differential production, mortality or sequestration of one gametocyte sex and selective advantages for the transmission of parasites.