Relationship between quantitative assessments of salivary buffering capacity and ion activity product for hydroxyapatite in relation to cariogenic potential
Relationship between quantitative assessments of salivary buffering capacity and ion activity product for hydroxyapatite in relation to cariogenic potential
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DOI：
10.1111/j.1834-7819.2008.00027.x
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发表时间：
2008-06-01
影响因子：
2.1
通讯作者：
Tagami, J.
中科院分区：
文献类型：
--
作者：
Aiuchi, H.;Kitasako, Y.;Tagami, J.
背景资料：羟基磷灰石离子活度积（Ip（HA））是反映唾液pH值、钙离子和磷酸根离子浓度的综合参数，决定了唾液对溶解性牙矿物质的饱和程度，本研究旨在评价唾液缓冲能力定量评价与Ip（HA）之间的关系，并与致龋潜力进行相关性分析。从33名患者收集刺激的全唾液，并使用手持式pH计测量样品的初始pH。然后用0.1 N HCl滴定样品以评价缓冲能力，并将样品分为三组（高、中和低）。测量样本中钙离子和磷酸根离子的浓度后，使用离子浓度和pH值计算Ip（HA）。使用Kruskal沃利斯和Mann-Whitney非参数检验分析三组之间的平均pH值、钙离子和磷酸根离子浓度以及log[Ip（HA）]的差异，p <0.05。经50 μ L HCl滴定后，各缓冲容量组的平均pH和Ip（HA）均有统计学差异。50 μ L盐酸滴定后，缓冲容量与log[Ip（HA）]之间存在良好的相关性。结论：盐酸滴定后唾液pH值的变化对Ip（HA）的速率有显著影响。
Background: The ion activity product for hydroxyapatite (Ip(HA)) is a comprehensive parameter reflecting pH, calcium and phosphate ion concentration in saliva which govern the degree of saturation with respect to the dissolving tooth mineral. The aim of this study was to evaluate the relationship between quantitative assessments of salivary buffering capacity and Ip(HA) in relation to cariogenic potential.Methods: Stimulated whole saliva was collected from 33 patients, and the initial pH of samples was measured using a hand-held pH meter. Then samples were titrated with 0.1 N HCl to evaluate buffering capacities and divided into three groups (high, medium and low). After measuring concentrations of calcium and phosphate ions in the samples, Ip(HA) was calculated using the values of the ion concentrations and pH. Differences in the mean pH values, the concentrations of calcium, phosphate ions and log[Ip(HA)] among three groups were analysed using the Kruskal Wallis and the Mann-Whitney non-parametric test, p < 0.05.Results: After HCl 50 mu L titration, there were statistical differences of the mean pH and Ip(HA) among each buffering capacity group. Moreover, after 50 mu L HCl titration, there was an excellent correlation between the buffer capacity and log[Ip(HA)].Conclusions: The pH change for saliva after HCl titration has a significant influence on the rate of Ip(HA).