In vitro study of bacterial degradation of ethyl glucuronide and ethyl sulphate
In vitro study of bacterial degradation of ethyl glucuronide and ethyl sulphate
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DOI：
10.1007/s00414-008-0229-3
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发表时间：
2008-09-01
影响因子：
2.1
通讯作者：
Halter, Claudia C.
中科院分区：
文献类型：
--
作者：
Baranowski, Stefanie;Serr, Annerose;Halter, Claudia C.
最近的研究表明，乙基葡萄糖醛酸（EtG）可以被细菌分解；同时迄今为止尚未观察到硫酸乙酯 (EtS) 的降解。在本研究中，进行了细菌菌落的体外实验。从尸检材料（肝脏、心血、尿液、腹水、心包液、胸膜液）中分离细菌（大肠杆菌、肺炎克雷伯菌、索氏梭菌）并测试 β-葡萄糖醛酸酶活性，并将三种细菌菌株添加到含有 EtG 和/或 EtS 的营养缺乏培养基中，并在 36 ℃下培养。 +/- 1 摄氏度。在长达 11 天的不同时间间隔后取样，并通过电喷雾电离串联质谱 (LC-ESI-MS/MS) 测定 EtG 和 EtS。 EtG被大肠杆菌和索氏梭菌降解——在3-4天的范围内发生完全降解——并且这些细菌表现出β-葡萄糖醛酸酶活性。 EtS 在孵化 11 天内没有受到影响。
Recent studies show that ethyl glucuronide (EtG) can be decomposed by bacteria; whilst so far no degradation of ethyl sulphate (EtS) has been observed. In the present study, in vitro experiments with bacterial colonies were performed. Bacteria (Escherichia coli, Klebsiella pneumoniae, Clostridium sordellii) were isolated from autopsy material (liver, heart blood, urine, ascites, pericardial fluid, pleural fluid) tested for beta-glucuronidase activity, and three bacterial strains were added to nutrient-deficient medium ontaining EtG and/or EtS and incubated at 36 +/- 1 degrees C. Samples were taken after various intervals up to 11 days, and EtG and EtS were determined by electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS). EtG was degraded by E. coli and C. sordellii-complete degradation occurred in the range of 3-4 days-and these bacteria exhibited beta-glucuronidase activity. EtS was not affected within 11 days of incubation.