Three-dimensional endomicroscopy of the human colon using optical coherence tomography.

Three-dimensional endomicroscopy of the human colon using optical coherence tomography.
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DOI:
10.1364/oe.17.000784
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发表时间:
2009-01-19
期刊:
影响因子:
3.8
通讯作者:
Fujimoto JG
Fujimoto JG
中科院分区:
物理与天体物理2区
文献类型:
--
作者:
Adler DC;Zhou C;Tsai TH;Schmitt J;Huang Q;Mashimo H;Fujimoto JG

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使用扫描源光学相干断层扫描(OCT)系统对人体胃肠道的三维(3D)内窥镜成像进行了活体演示。来自7名接受诊断或治疗性结肠镜检查的志愿者获得了结肠、直肠和肛缘正常和病理区域的3D数据集。3D-OCT通过使用任意方向的虚拟横断面图像和面部投影图像来可视化组织结构形态,从而实现高分辨率的内窥镜检查。在~180mm2的视场范围内,获得了6μm的组织轴向像分辨率,成像范围为1.6 mm。以62 kHz的扫频提供180 nm的调谐范围的傅立叶域锁模(FDML)激光器被用作系统光源。这项临床先导性研究展示了3D-OCT在区分正常和病理结直肠组织、评估内窥镜治疗和愈合进程方面的潜力。
Three-dimensional (3D) endomicroscopy imaging of the human gastrointestinal tract is demonstrated in vivo using a swept source optical coherence tomography (OCT) system. 3D datasets of normal and pathologic regions of the colon, rectum, and anal verge were obtained from seven volunteers undergoing diagnostic or therapeutic colonoscopy. 3D-OCT enables high resolution endomicroscopy examination through visualization of tissue architectural morphology using virtual cross-sectional images with arbitrary orientations as well as en face projection images. Axial image resolutions of 6 μm in tissue are obtained over a ~180 mm2 field with an imaging range of 1.6 mm. A Fourier domain mode locked (FDML) laser providing a tuning range of 180 nm at a sweep rate of 62 kHz is used as the system light source. This clinical pilot study demonstrates the potential of 3D-OCT for distinguishing normal from pathologic colorectal tissue, assessing endoscopic therapies and healing progression.
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