Ralstonia solanacearum elicitor RipX Induces Defense Reaction by Suppressing the Mitochondrial atpA Gene in Host Plant
Ralstonia solanacearum elicitor RipX Induces Defense Reaction by Suppressing the Mitochondrial atpA Gene in Host Plant
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青枯雷尔斯顿菌诱导子 RipX 通过抑制宿主植物线粒体 atpA 基因诱导防御反应
DOI：
10.3390/ijms21062000
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发表时间：
2020-03
影响因子：
5.6
通讯作者：
Zou Huasong
中科院分区：
文献类型：
--
作者：
Sun Tingyan;Wu Wei;Wu Haoxiang;Rou Wei;Zhou Yinghui;Zhuo Tao;Fan Xiaojing;Hu Xun;Zou Huasong
青枯雷尔氏菌的RipX通过III型分泌系统转运到宿主细胞中，并作为Harpin样蛋白诱导烟草植物的过敏反应。与RipX诱导的信号转导相关的分子事件尚未完全阐明。这项工作报告了RipX的瞬时表达诱导了本氏烟草的黄化表型，并激活了防御反应。利用酵母双杂交和分裂荧光素酶互补技术，鉴定了线粒体ATP合成酶F1亚基α（ATPA）是N.本萨米亚纳虽然在线粒体中发现了一定比例的YFP-ATPA融合，能够定位于细胞膜，细胞质和细胞核。RFP-RipX融合蛋白存在于细胞膜和细胞质中。此外，ATPA与RipX在体内的细胞膜和细胞质中的相互作用。atpA基因的沉默对RipX诱导的黄化表型的出现没有影响。但是，沉默植株提高了对白叶枯病菌的抗性。青枯菌属此外，qRT-PCR和启动子GUS融合实验表明，RipX的表达显著降低了atpA的转录水平。这些结果表明，RipX通过抑制atpA基因的转录，诱导烟草的防卫反应。本萨米亚纳
RipX of Ralstonia solanacearum is translocated into host cells by a type III secretion system and acts as a harpin-like protein to induce a hypersensitive response in tobacco plants. The molecular events in association with RipX-induced signaling transduction have not been fully elucidated. This work reports that transient expression of RipX induced a yellowing phenotype in Nicotiana benthamiana, coupled with activation of the defense reaction. Using yeast two-hybrid and split-luciferase complementation assays, mitochondrial ATP synthase F1 subunit α (ATPA) was identified as an interaction partner of RipX from N. benthamiana. Although a certain proportion was found in mitochondria, the YFP-ATPA fusion was able to localize to the cell membrane, cytoplasm, and nucleus. RFP-RipX fusion was found from the cell membrane and cytoplasm. Moreover, ATPA interacted with RipX at both the cell membrane and cytoplasm in vivo. Silencing of the atpA gene had no effect on the appearance of yellowing phenotype induced by RipX. However, the silenced plants improved the resistance to R. solanacearum. Moreover, qRT-PCR and promoter GUS fusion experiments revealed that the transcript levels of atpA were evidently reduced in response to expression of RipX. These data demonstrated that RipX exerts a suppressive effect on the transcription of atpA gene, to induce defense reaction in N. benthamiana.
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影响因子：
7.2
作者：
Belbahri, L;Boucher, C;Keller, H
通讯作者：
Keller, H
影响因子：
3.5
作者：
Poueymiro, Marie;Cunnac, Sebastien;Genin, Stephane
通讯作者：
Genin, Stephane
DOI：
10.1016/s0021-9258(18)42529-x
发表时间：
1992-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Ackerman;J. Martín;A. Tzagoloff
通讯作者：
S. Ackerman;J. Martín;A. Tzagoloff
影响因子：
6.4
作者：
Mukaihara T;Hatanaka T;Nakano M;Oda K
通讯作者：
Oda K
影响因子：
3.6
作者：
Guéneron, M;Timmers, ACJ;Arlat, M
通讯作者：
Arlat, M