Unique tissue distribution of a mouse macrophage C-type lectin.
Unique tissue distribution of a mouse macrophage C-type lectin.
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小鼠巨噬细胞 C 型凝集素的独特组织分布。
DOI：
10.1093/glycob/7.1.137
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发表时间：
1997
期刊：
影响因子：
4.3
通讯作者：
T. Irimura
中科院分区：
文献类型：
--
作者：
S. Mizuochi;Y. Akimoto;Y. Imai;H. Hirano;T. Irimura
我们使用针对巨噬细胞半乳糖/N-乙酰半乳糖胺特异性C型凝集素的鼠单抗(mAbLOM-14)检测了这种凝集素在小鼠组织中的表达。免疫印迹法检测组织洗涤剂提取物中单抗LOM-14的结合。结果表明，该单抗与结构同源的小鼠肝凝集素无交叉反应。经亲和分离和免疫化学检测，巨噬细胞凝集素广泛分布于小鼠各组织中。例外的是脑、肝、肾、小肠和外周血液。尽管存在表达巨噬细胞标志物的细胞，但这些器官的提取物在mAbLOM-14结合上表现出非常微弱的信号。在皮肤提取液中观察到的信号最强，表明皮肤中有大量表达这种凝集素的细胞。切片的免疫组织化学染色进一步研究了含有这种凝集素的组织。细胞分布于结缔组织和间质，尤其是真皮和皮下层。定位于皮肤上皮(表皮)或我们所检查的其他上皮中的细胞未被染色。心肌和骨骼肌组织中也显示了单抗LOM-14染色的细胞在血管周围的定位。免疫电子显微镜显示这种凝集素沿粗面内质网存在。总之，半乳糖/N-乙酰氨基半乳糖的C型凝集素在小鼠体内的分布是独一无二的。结缔组织的特异性分布应该为这种凝集素的生物学作用提供重要的信息。
We examined mouse tissue for the expression of macrophage galactose/N-acetylgalactosamine-specific C-type lectin using a rat monoclonal antibody (mAb) specific for this lectin (mAb LOM-14). The binding of mAb LOM-14 was detected in detergent extracts from tissue by means of immunoblotting analysis. It was shown that this mAb did not cross-react with mouse hepatic lectins, a structural homologue. The macrophage lectin was widely distributed among various mouse tissues as judged by the affinity isolation followed by the immunochemical detection. The exceptions were brain, liver, kidney, small intestine, and peripheral blood. Extracts from these organs exhibited, at best, very weak signals upon mAb LOM-14 binding, despite the presence of cells expressing macrophage markers. The most intense signal was observed in the extract from skin, suggesting that cells expressing this lectin are abundant in skin. The tissues shown to contain this lectin were further investigated by immunohistochemical staining of the sections. Cells were distributed in the connective tissue and in the interstice, particularly the dermis and subcutaneous layer of skin. Cells localized in the epithelium of skin (epidermis) or other epithelia that we examined were not stained. Perivascular localization of cells stained with mAb LOM-14 was also demonstrated in cardiac and skeletal muscle tissues. Immunoelectron microscopy revealed the presence of this lectin along the rough endoplasmic reticulum. In conclusion, the distribution of C-type lectin specific for galactose/N-acetylgalactosamine in mice was unique. The connective tissue-specific distribution should provide important information on the biological role of this lectin.
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影响因子：
4.4
作者：
M. Ho;T. Springer
通讯作者：
M. Ho;T. Springer
大鼠 Kupffer 细胞特有的碳水化合物结合受体 cDNA 的分子克隆和测序。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hoyle,GW;Hill,RL
通讯作者：
Hill,RL
小鼠脱唾液酸糖蛋白受体 cDNA 序列：哺乳动物进化过程中受体基因的保守。
DOI：
10.1016/0167-4781(90)90216-o
发表时间：
1990
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Sanford,JP;Doyle,D
通讯作者：
Doyle,D
慢性心脏排斥：通过差异 mRNA 显示鉴定移植心脏中五个上调基因。
DOI：
10.1073/pnas.91.14.6463
发表时间：
1994
影响因子：
11.1
作者：
Utans,U;Liang,P;Wyner,LR;Karnovsky,MJ;Russell,ME
通讯作者：
Russell,ME
基于 GaN 量子阱的 249 nm 深紫外激光器
影响因子：
7
作者：
Shan, Maocheng;Zhang, Yi;Li, Xiaohang
通讯作者：
Li, Xiaohang