ALKALINE CELLULASES FOR LAUNDRY DETERGENTS - PRODUCTION BY ALKALOPHILIC STRAINS OF BACILLUS AND SOME PROPERTIES OF THE CRUDE ENZYMES
ALKALINE CELLULASES FOR LAUNDRY DETERGENTS - PRODUCTION BY ALKALOPHILIC STRAINS OF BACILLUS AND SOME PROPERTIES OF THE CRUDE ENZYMES
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DOI：
10.1080/00021369.1990.10869922
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发表时间：
1990-01-01
期刊：
影响因子：
--
通讯作者：
ITO, S
中科院分区：
文献类型：
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作者：
SHIKATA, S;SAEKI, K;ITO, S
分离到3株嗜碱芽孢杆菌KSM-19、KSM-和KSM-520，它们的碱性纤维素酶适合用作提高洗涤剂产品效率的添加剂。它们的活性完全不受金属离子或洗涤产品的各种成分的抑制，如表面活性剂、螯合剂和蛋白酶。该酶制剂对羧甲基纤维素酶活性较强，最适pH值为8.5~9.5，最适温度约为50℃。C.菌株在初始pH为8.5-9.5时生长最快，在中性pH时有少量生长。碱性纤维素酶的产生需要羧甲基纤维素酶的存在，并受分解代谢抑制和诱导的机制控制。两株菌株KSM-19和KSM-产生碱性地衣多糖水解酶。
Three strains of alkalophilic Bacillus, KSM-19, KSM-64 and KSM-520, were isolated which produced alkaline cellulases suitable as additives for improving the efficiency of detergent products. Their activities were not inhibited at all by metal ions or various components of laundry products, such as surfactants, chelating agents and proteinases. The enzyme preparations showed strong activities toward carboxymethyl cellulose, the optimum pHs being 8.5-9.5 and the optimum temperatures about 50.degree. C. Maximum growth of the isolates was observed at an initial pH of 8.5-9.5; slight growth occurred at neutral pH. Production of the alkaline cellulases required the presence of carboxymethyl cellulose, and it was controlled by a mechanism involving catabolite repression and induction. Two strains, KSM-19 and KSM-64, produced alkaline lichenan-hydrolyzing enzymes.