Preferential expression of HSC70 heat shock mRNA in gerbil heart after transient brain ischemia.
Preferential expression of HSC70 heat shock mRNA in gerbil heart after transient brain ischemia.
复制标题
短暂脑缺血后沙鼠心脏中 HSC70 热休克 mRNA 的优先表达。
DOI：
10.1006/jmcc.1993.1126
复制
发表时间：
1993
影响因子：
5
通讯作者：
S. Isoyama
中科院分区：
文献类型：
--
作者：
K. Abe;T. H. Lee;M. Aoki;Y. Nitta;S. Isoyama
在沙土鼠短暂性前脑缺血10min后，观察了大脑皮层、小脑、心、肺、肾、肝等组织中热休克蛋白(HSP)70和热休克相关蛋白(HSC)70的表达。HSP70mRNA在小脑、肺和肾脏中有少量表达，但在心脏和肝脏中不表达。大脑皮层也有极少量的HSP70基因表达。HSC70mRNA在所有器官中正常表达，但数量不同。脑缺血8h后，大脑皮层、肺、肾组织中HSP70mRNA表达水平升高。HSC70基因在各脏器中的表达水平也均升高。而心脏组织中HSC70mRNA的表达显著增加。短暂性脑缺血引起随后的体温升高。沙土鼠在无脑缺血条件下进行人工加温处理后，HSP70和HSC70基因表达水平也明显升高。而脑缺血后心脏组织HSC70mRNA的表达水平明显高于高温组。这些结果表明，在短暂性前脑缺血后，心脏优先诱导HSC70mRNA的表达，这不仅仅是由于随后的高温。
Inductions of mRNAs for heat shock protein (HSP) 70 and heat shock cognate protein (HSC) 70 were examined in the cerebral cortex, cerebellum, heart, lung, kidney, and liver of gerbils after a 10-min transient forebrain ischemia. HSP70 mRNA was normally expressed in a small amount in the cerebellum, lung, and kidney, but was not expressed in the heart or liver in a detectable amount. A very small amount of HSP70 mRNA was also present in the cerebral cortex. HSC70 mRNA was normally present in all the organs examined with a variety in the amount. Eight hours after the cerebral ischemia, the level of HSP70 mRNA increased in the cerebral cortex, lung, and kidney. HSC70 mRNA levels also increased in all the organs. However, the increase of HSC70 mRNA was remarkable in the heart. Transient cerebral ischemia caused subsequent hyperthermia. Treatment of gerbils with an artificial hyperthermia without cerebral ischemia increased the HSP70 and HSC70 mRNA levels as well. However, the HSC70 mRNA level in the heart after cerebral ischemia was much higher than that in the case with hyperthermic treatment. These results suggest that HSC70 mRNA was preferentially induced in the heart after transient forebrain ischemia that was not only due to the subsequent hyperthermia.