Isolation and studies of the granules of the amebocytes of Limulus polyphemus, the horseshoe crab
Isolation and studies of the granules of the amebocytes of Limulus polyphemus, the horseshoe crab
复制标题
鲎变形细胞颗粒的分离与研究
DOI：
10.1002/jcp.1040860310
复制
发表时间：
1975
影响因子：
5.6
通讯作者：
R. Holme
中科院分区：
文献类型：
--
作者：
E. Mürer;J. Levin;R. Holme
通过破碎从已吸入2mM普萘洛尔的血液中获得的细胞，从鲎（鲎）的变形细胞的细胞质中分离颗粒。随后通过含有肝素的蔗糖梯度离心纯化颗粒。通过在蒸馏水中冷冻和解冻颗粒制剂来制备颗粒的提取物。
Granules were isolated from the cytoplasm of the amebocytes of Limulus polyphemus, the horseshoe crab, by disruption of cells obtained from blood which had been drawn into 2 mM propranolol. The granules subsequently were purified by centrifugation through a sucrose gradient that contained heparin. Extracts of the granules were prepared by freezing and thawing the granule preparations in distilled water.