Suppression of VEGF-mediated autocrine and paracrine interactions between prostate cancer cells and vascular endothelial cells by soy isoflavones
Suppression of VEGF-mediated autocrine and paracrine interactions between prostate cancer cells and vascular endothelial cells by soy isoflavones
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DOI：
10.1016/j.jnutbio.2006.08.006
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发表时间：
2007-06-01
影响因子：
5.6
通讯作者：
Clinton, Steven K.
中科院分区：
文献类型：
--
作者：
Guo, Yanping;Wang, Shihua;Clinton, Steven K.
血管生成是前列腺癌发生发展的重要过程。血管内皮生长因子（VEGF）被认为是前列腺癌发生过程中血管生成的关键调节因子。我们已经报道了饮食豆制品在动物模型中抑制前列腺肿瘤的进展，与肿瘤微血管密度的降低有关。本研究的目的是利用体外系统研究染料木素（主要的大豆异黄酮）的潜在抗血管生成机制。染料木素（550 μ M）在添加VEGF刺激的对照培养基或缺氧暴露的PC-3前列腺腺癌细胞条件培养基中培养时，显著抑制人脐静脉内皮细胞（HUVECs）的生长。这些体外研究表明，5-10 μ M染料木素(P
Angiogenesis is an essential process involved in the development and progression of prostate cancer. Vascular endothelial growth factor (VEGF) is hypothesized to be a critical regulator of angiogenesis during prostate carcinogenesis. We have reported that dietary soy products inhibit prostate tumor progression in animal models, in association with a reduction in tumor microvessel density. The goal of the present study is to investigate potential antiangiogenic mechanisms of genistein, the major soy isoflavone, using in vitro systems. Genistein (550 mu M) significantly inhibited the growth of human umbilical vein endothelial cells (HUVECs) in control media when stimulated by supplemental VEGF or when cultured in hypoxia-exposed PC-3 prostate adenocarcinoma, cell conditioned media. These in vitro studies suggest detectable inhibitory effects by 5-10 mu M genistein (P