High UBCH10 protein expression as a marker of poor prognosis in esophageal squamous cell carcinoma.
High UBCH10 protein expression as a marker of poor prognosis in esophageal squamous cell carcinoma.
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发表时间：
2014-02
影响因子：
2
通讯作者：
Akira Matsumoto;Y. Ishibashi;M. Urashima;N. Omura;K. Nakada;Katsunori Nishikawa;Atsuo Shida;K. Takada;H. Kashiwagi;K. Yanaga
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作者：
Akira Matsumoto;Y. Ishibashi;M. Urashima;N. Omura;K. Nakada;Katsunori Nishikawa;Atsuo Shida;K. Takada;H. Kashiwagi;K. Yanaga
背景与目的泛素结合酶H10（UBCH 10）是细胞周期由中期向后期转换所必需的。因此，我们研究其在食管癌病变中的表达水平是否影响食管鳞状细胞癌患者的预后。材料与方法采用UBCH 10抗体对121例食管鳞癌石蜡包埋组织进行免疫组化染色。结果UBCH 10在癌组织和不典型增生组织中均有表达，而在正常组织中无表达。根据表达对患者进行分组：高（N=33）或低（N=88），取决于染色模式。两组在淋巴管浸润、转移淋巴结数量、TNM分期和生存期方面存在显著差异：高表达组的50%生存率为2.3年，而低表达组为9.9年（p<0.0001）。即使使用考克斯比例风险模型对0期至IV期进行多变量调整，属于高表达组的患者预后不良（风险比=2.5; 95%置信区间=1.3-4.5; p=0.004）。结论UBCH 10蛋白高表达是食管鳞癌预后不良的标志。
BACKGROUND/AIM Ubiquitin-conjugating enzyme H10 (UBCH10) is required in the cell-cycle transition from metaphase to anaphase. Therefore, we investigated whether its expression level in cancerous esophageal lesions affected prognosis of patients with esophageal squamous-cell carcinoma. MATERIALS AND METHODS Paraffin-embedded tissue samples from 121 patients with esophageal squamous cell carcinoma were stained with antibody to UBCH10 for immunohistochemical analysis. RESULTS UBCH10 was expressed in cancerous and dysplastic lesions, but not in normal tissue. Patients were grouped according to expression: High (N=33) or low (N=88), depending on the staining pattern. There were significant differences between the groups in terms of invasion into lymphatic vessels, number of metastatic lymph nodes, TNM classification, and stages, as well as in survival: the 50% survival rate in the high expression group was 2.3 years, whereas it was 9.9 years for the low-expression group (p<0.0001). Even with multivariate adjusting for stage 0 to stage IV using the Cox proportional hazard model, patients belonging to the high-expression group had a poor prognosis (Hazard ratio=2.5; 95% Confidence Interval=1.3-4.5; p=0.004). CONCLUSION High protein expression of UBCH10 is a marker of poor prognosis in esophageal squamous cell carcinoma.