Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein
Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein
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DOI：
10.1074/jbc.m108665200
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发表时间：
2002-04-05
影响因子：
4.8
通讯作者：
McCaffrey, MW
中科院分区：
文献类型：
--
作者：
Lindsay, AJ;Hendrick, AG;McCaffrey, MW
Rab 4和Rab 11是属于Ras超家族的小GTP酶。它们都作为调节剂沿着受体再循环途径。我们已经确定了一种新的80 kDa的蛋白质，特别是与GTP结合的构象Rab 4的相互作用，随后的工作表明，它也与Rab 11强烈相互作用。我们将这种蛋白质命名为Rab偶联蛋白（RCP）。RCP主要是膜结合的，并在所有检测的细胞系和组织中表达。它与早期内体标记物包括Rab 4和Rab 11以及转铁蛋白受体共定位。过表达的RCP，其中包含Rab 4和Rab 11相互作用的结构域的羧基末端区域，结果在一个显着的转铁蛋白隔室。此外，该突变体的表达引起内体再循环的显著减少，而不影响定量测定中的配体摄取或降解。RCP是Rip 11的同源物，因此属于最近描述的Rab 11-FIP家族。
Rab4 and Rab11 are small GTPases belonging to the Ras superfamily. They both function as regulators along the receptor recycling pathway. We have identified a novel 80-kDa protein that interacts specifically with the GTP-bound conformation of Rab4, and subsequent work has shown that it also interacts strongly with Rab11. We name this protein Rab coupling protein (RCP). RCP is predominantly membrane-bound and is expressed in all cell lines and tissues tested. It colocalizes with early endosomal markers including Rab4 and Rab11 as well as with the transferrin receptor. Overexpression of the carboxyl-terminal region of RCP, which contains the Rab4- and Rab11-interacting domain, results in a dramatic tubulation of the transferrin compartment. Furthermore, expression of this mutant causes a significant reduction in endosomal recycling without affecting Iigand uptake or degradation in quantitative assays. RCP is a homologue of Rip11 and therefore belongs to the recently described Rab11-FIP family.