Aberrantly expressed long noncoding RNAs in recurrent implantation failure: A microarray related study
Aberrantly expressed long noncoding RNAs in recurrent implantation failure: A microarray related study
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DOI：
10.1080/19396368.2017.1310329
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发表时间：
2017-01-01
影响因子：
2.4
通讯作者：
Shi, Cheng
中科院分区：
文献类型：
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作者：
Fan, Li-juan;Han, Hong-jing;Shi, Cheng
长链非编码RNA（lncRNA）是一类长度超过200个核苷酸的非编码RNA。由于其低表达和非蛋白质编码的特点，长期以来被认为是转录噪声。最近发表的报告表明，lncRNA几乎涉及人类生物学的各个方面。我们的目的是分析复发性着床失败（RIF）妇女的子宫内膜lncRNA表达模式，并预测失调的lncRNA转录本基因的功能。在着床窗口期采集子宫内膜样本（24例）（14例RIF女性和10例胚胎移植后受孕的女性）。对于微阵列研究，选择7个RIF子宫内膜和5个对照子宫内膜，并对其余子宫内膜样品进行定量实时PCR（RT-qPCR）以验证微阵列结果。通过lncRNA-mRNA共表达分析、GO分析、KEGG分析、lncRNA-transcript factor（TF）分析等方法，对表达异常的lncRNA转录本的基因功能进行分析。与对照组相比，我们共检测到197个lncRNA转录本在RIF子宫内膜中失调。通过RT-qPCR验证了8种选择的lncRNA转录物的相对表达水平，并且与微阵列结果一致。GO和KEGG分析显示，共表达的mRNA转录本参与了可能影响子宫内膜容受性的途径，如细胞粘附。lncRNA靶标预测提供了失调lncRNA转录物的潜在TF靶标。我们的研究结果表明，RIF子宫内膜的lncRNA表达谱与正常接受性子宫内膜不同，提示lncRNA可能调节子宫内膜的接受性。缩略语：GO：基因肿瘤学; GF：生长因子; KEGG：京都基因和基因组百科全书; lncRNA：长链非编码RNA; PCA 3：前列腺癌抗原3; RT-qPCR：定量实时PCR; RIF：复发性着床失败; STK：丝氨酸/苏氨酸激酶; TF：转录因子; WOI：着床窗
Long noncoding RNAs (lncRNAs) are a class of noncoding RNAs longer than 200 nucleotides. They were long regarded as transcription noise for their low expression and non-protein coding features. Recent published reports indicate that lncRNAs are involved in virtually every aspect of human biology. We aimed to profile the endometrial lncRNA expression pattern in women with recurrent implantation failure (RIF) and predict the function of the genes of the dysregulated lncRNA transcripts. Endometrial samples (24) were collected during window of implantation (14 RIF women and 10 women who conceived after embryo transfer). For the microarray study, 7 RIF endometrium and 5 control endometrium were selected, and quantitative real-time PCR (RT-qPCR) was performed on the rest of the endometrial samples to validate the microarray results. After that, lncRNA-mRNA co-expression analysis, GO analysis, KEGG analysis, and lncRNA-transcript factor (TF) analysis were carried out to analyze the gene functions of the dysregulated lncRNA transcripts. We detected a total of 197 lncRNA transcripts that were dysregulated in RIF endometrium compared with the control group. The relative expression levels of eight selected lncRNA transcripts were validated by RT-qPCR and were in accordance with the microarray outcomes. GO and KEGG analyses revealed that the coexpressed mRNA transcripts were involved in pathways that may affect endometrial receptivity such as cell adhesion. The lncRNA target predictions provided potential TF targets of the dysregulated lncRNA transcripts. Our results indicate that lncRNA expression profiles of RIF endometrium were different from that of normal receptive endometrial, suggesting that lncRNAs may regulate endometrial receptivity.Abbreviations: GO: Gene Oncology; GFs: growth factors; KEGG: Kyoto Encyclopedia of Genes and Genomes; lncRNAs: long noncoding RNAs; PCA3: prostate cancer antigen 3; RT-qPCR: quantitative real-time PCR; RIF: recurrent implantation failure; STK: serine/threonine kinase; TF: transcription factor; WOI: window of implantation