Comparison of Trypanosoma cruzi infection in dogs inoculated with blood or metacyclic trypomastigotes of Berenice-62 and Berenice-78 strains via intraperitoneal and conjunctival routes
Comparison of Trypanosoma cruzi infection in dogs inoculated with blood or metacyclic trypomastigotes of Berenice-62 and Berenice-78 strains via intraperitoneal and conjunctival routes
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DOI：
10.1590/s0037-86822002000400010
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发表时间：
2002-08-01
影响因子：
2
通讯作者：
Lana, Marta de
中科院分区：
文献类型：
--
作者：
Bahia, Maria Terezinha;Tafuri, Washington Luiz;Lana, Marta de
本文旨在通过对比克氏锥虫Berenice-62和Berenice-78菌株，验证接种来源（血液或后循环锥鞭毛体）和接种途径（腹膜内或结膜内）对犬克氏锥虫感染病程的影响。所有腹膜内接种的狗均被感染，与所使用的克氏锥虫菌株和锥鞭毛体来源无关。当两种菌株的后循环锥鞭毛体通过结膜途径接种时，也观察到高水平的感染性。然而，当通过结膜途径接种血液锥鞭毛体时，接种两种菌株的狗的感染率明显较低。在通过结膜途径感染变环锥鞭毛体的动物中，寄生虫血症显着较高，与所使用的克氏锥虫菌株无关。所有感染Berenice-78株的动物均表现出严重的急性心肌炎。另一方面，感染Berenice-62的动物只有在通过腹膜内途径感染后循环锥鞭毛体时才表现出严重的急性心肌炎。结果表明，即使使用相同菌株的寄生虫，接种物的来源和接种途径也会显着影响脊椎动物宿主中克氏锥虫的感染进化。
This paper aimed to verify the influence of the inoculum source (blood or metacyclic trypomastigote) and the route of inoculation (intraperitoneal or conjunctival) on the course of T. cruzi infection in dogs, using comparatively the T. cruzi strains Berenice-62 and Berenice-78. All dogs inoculated intraperitoneally became infected independently of the T. cruzi strain and source of trypomastigotes used. High level of infectivity was also observed when metacyclic trypomastigotes of both strains were inoculated by conjunctival route. However, when blood trypomastigotes were inoculated by conjunctival route the percentages of infectivity were significantly lower in dogs inoculated with both strains. Parasitaemia was significantly higher in animals infected with metacyclic trypomastigotes via the conjunctival route independently of the T. cruzi strain used. All animals infected with Berenice-78 strain showed severe acute myocarditis. On the other hand, animals infected with Berenice-62 showed severe acute myocarditis only when infected with metacyclic trypomastigote, via the intraperitoneal route. The results suggest that the source of the inoculum and the route of inoculation remarkably influence the evolution of the infection for the T. cruzi in the vertebrate host even when the same strain of the parasite is used.