Isolation and characterization of a R2R3-MYB transcription factor gene related to anthocyanin biosynthesis in the spathes of Anthurium andraeanum (Hort.)
Isolation and characterization of a R2R3-MYB transcription factor gene related to anthocyanin biosynthesis in the spathes of Anthurium andraeanum (Hort.)
复制标题
红掌花苞中与花青素生物合成相关的 R2R3-MYB 转录因子基因的分离和表征
DOI：
10.1007/s00299-016-2025-8
复制
发表时间：
2016-10-01
影响因子：
6.2
通讯作者：
Yin, Junmei
中科院分区：
文献类型：
--
作者：
Li, Chonghui;Qiu, Jian;Yin, Junmei
从红掌（Anthuriumandraeanum（Hort.）花焰苞着色是红掌（Anthuriumandraeanum（Hort.）这一特性主要是由花色苷贡献的。R2 R3-MYB转录因子是植物花色素苷合成的重要调控因子。本文报道了R2 R3-MYB转铁蛋白家族第6亚组成员AaMYB 2的鉴定和特性，该基因与A. andraeanum。AaMYB 2是一个具有正转录活性的核定位蛋白，在红色佛焰苞中显著表达。AaMYB 2在烟草中的异位表达导致花色素苷的积累和早期和晚期花色素苷途径基因的上调，特别是NtDFR，NtANS和NtUFGT，以及叶片中的内源TF基因NtAn 2和NtAn 1。在“热带”和“Vitara”的红色佛焰苞发育过程中，AaMYB 2的表达与花色素苷积累密切相关，并与AaCHS、AaF 3 H和AaANS共表达，后两者被认为是控制红掌佛焰苞颜色遗传的TF基因R位点的潜在靶基因。此外，AaMYB 2基因在不同花色品种中的转录水平表明，AaMYB 2基因在红色、粉红色和紫色品种的佛焰苞中大量表达，而在白色和绿色品种的佛焰苞中几乎不表达。此外，AaMYB 2也表现出较高的表达在新发育的叶片时，花青素的生物合成活跃。综上所述，AaMYB 2与花焰苞和叶片中的花青素苷生物合成呈正相关，并且似乎调节AaF 3 H、AaANS和可能的AaCHS的表达。
A R2R3-MYB gene AaMYB2 was isolated from Anthurium andraeanum (Hort.) and was functionally characterized to be a positive transcriptional regulator for anthocyanin biosynthesis in the spathes and leaves.Spathe coloration is an important Anthurium andraeanum (Hort.) characteristic, which is mainly contributed by anthocyanins. R2R3-MYB transcription factors (TFs) are important regulators of anthocyanin biosynthesis in plants. Here we describe the identification and characterization of AaMYB2, a member in subgroup 6 of the R2R3-MYB TFs family, which correlated with anthocyanin biosynthesis in A. andraeanum. AaMYB2 was a nuclear-localization protein with positive transcriptional activity, and prominently expressed in the red spathes. Ectopic expression of AaMYB2 in tobacco led to anthocyanin accumulation and up-regulation of the early and late anthocyanin pathway genes, particularly NtDFR, NtANS, and NtUFGT, and the endogenous TF genes NtAn2 and NtAn1 in leaves. In the developing red spathes of 'Tropical' and 'Vitara', the expression of AaMYB2 was closely linked to anthocyanin accumulation, and co-expressed with AaCHS, AaF3H, and AaANS, the latter two of which were regarded as the potential targets of the R locus encoding a TF controlling spathe colors inheritance in anthurium. In addition, the transcription level of AaMYB2 in various cultivars with different color phenotypes showed that AaMYB2 was drastically expressed in the spathes from the red, pink, and purple cultivars, but hardly detected in the spathes from the white and green ones. Besides, AaMYB2 also showed higher expression in newly developmental leaves when anthocyanin was actively biosynthesized. Taken together, AaMYB2 positively related to anthocyanin biosynthesis in anthurium spathes and leaves, and appeared to regulate the expression of AaF3H, AaANS, and possibly AaCHS.