Alternative 5′-untranslated first exons of the mouse Cyp19A1 (aromatase) gene
Alternative 5′-untranslated first exons of the mouse Cyp19A1 (aromatase) gene
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DOI：
10.1016/j.jsbmb.2009.03.010
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发表时间：
2009-07-01
影响因子：
4.1
通讯作者：
Boon, Wah Chin
中科院分区：
文献类型：
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作者：
Chow, Jenny D. Y.;Simpson, Evan R.;Boon, Wah Chin
人类芳香化酶基因（CYP 19 A1）有11个组织特异性非翻译的第一外显子，而在小鼠Cyp 19 A1中仅描述了3个，即脑、卵巢和睾丸特异性外显子1。本研究旨在阐明小鼠Cyp 19 A1基因的完整结构。我们在小鼠骨骼、主动脉、下丘脑、脂肪、性腺和胎盘中检测到芳香化酶的转录本，但在未产的乳腺脂肪垫中没有检测到。针对人CYP 19 A1的BestFit算法分析已经鉴定了小鼠Cyp 19 A1上游的10个推定的第一外显子。基于这些推测的序列，我们能够设计特异性引物进行RT-PCR，并首次检测到，外显子1.4和1.3的存在下，在小鼠脂肪和性腺，分别。这些是小鼠Cyp 19 A1的新型5 'UTR。用RT-PCR和5' RACE方法证实了外显子If在下丘脑和近端外显子P2在卵巢中的表达。睾丸特异性外显子I开始217 bp进一步上游比以前报道的。在小鼠组织中未检测到推定的外显子2a、1.5、1.7、1.6和1.2。因此，我们发现小鼠Cyp 19 A1包含比以前认为的更多的组织特异性第一外显子，并显示出与人CYP 19 A1相似的基因组结构。(C)2009爱思唯尔有限公司版权所有。
The human aromatase gene (CYP19A1) has eleven tissue-specific untranslated first exons, while only three have been described in the mouse Cyp19A1 namely brain-, ovary- and testis-specific exons 1. The present study aims to elucidate the complete structure of the mouse Cyp19A1 gene. We detected aromatase transcripts in mouse bone, aorta, hypothalamus, adipose, gonads and placenta, but not nulliparous mammary fat pad. BestFit algorithm analysis against the human CYP19A1 has identified ten putative first exons upstream of mouse Cyp19A1. Based on these putative sequences, we were able to design specific primers for RT-PCR and detected for the first time, the presence of exons 1.4 and 1.3 in murine fat and gonads, respectively. These are novel 5'UTRs of mouse Cyp19A1. Using RT-PCR and 5' RACE, we confirmed the expression of exon If in the hypothalamus and proximal exon P2 in the ovary. The testis-specific exon I begins 217bp further upstream than previously reported. Putative exons 2a, 1.5, 1.7, 1.6 and 1.2 were not detected in mouse tissues. Therefore, we showed that mouse Cyp19A1 contains more tissue-specific first exons than previously thought and displays a similar genomic organization to human CYP19A1. (C) 2009 Elsevier Ltd. All rights reserved.