Simultaneous sorting of multiple bacterial targets using integrated Dielectrophoretic-Magnetic Activated Cell Sorter
Simultaneous sorting of multiple bacterial targets using integrated Dielectrophoretic-Magnetic Activated Cell Sorter
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DOI：
10.1039/b903950c
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Soh, H. Tom
中科院分区：
文献类型：
--
作者：
Kim, Unyoung;Soh, H. Tom
从复杂的样品混合物中快速准确地分选多种类型的生物靶标（如分子、病毒、细菌或哺乳动物细胞）的能力是广泛的诊断和治疗策略的重要组成部分。然而，用于细胞分离的大多数当前选择方法在通量方面受到限制，如荧光辅助细胞分选（FACS）的情况，或者仅允许通过单个参数标记的靶标的二元分离，如磁激活细胞分选（MACS）。我们在这里报告了集成的Differentistic-Magnetic Activated Cell Sorter（iDMACS），这是一种集成平台，在单个微流体设备中结合了两种不同的力场，用于高效的多目标分离。我们描述了iDMACS设备的基本物理和设计，并证明了在单轮分离后具有超过95%纯度的多种细菌靶细胞类型的900倍富集。
The ability to rapidly and accurately sort multiple types of biological targets-such as molecules, viruses, bacteria or mammalian cells-from complex sample mixtures is an essential component for a wide range of diagnostic and therapeutic strategies. However, most current selection methods for cell separation are either limited with regard to throughput, as is the case for Fluorescence Assisted Cell Sorting (FACS), or else only allow binary separation of targets that have been labeled via a single parameter, such as Magnetic Activated Cell Sorting (MACS). We report here the integrated Dielectrophoretic-Magnetic Activated Cell Sorter (iDMACS), an integrated platform that combines two different force fields in a single microfluidic device for highly efficient multi-target separation. We describe the underlying physics and design of the iDMACS device and demonstrate similar to 900-fold enrichment of multiple bacterial target cell types with over 95% purity after a single round of separation.