Synthesis and property of DNA labeled with fluorescent acridone.
Synthesis and property of DNA labeled with fluorescent acridone.
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DOI：
10.1093/nass/nrl072
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发表时间：
2006-01-01
期刊：
影响因子：
--
通讯作者：
Sawai, Hiroaki
中科院分区：
文献类型：
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作者：
Hasegawa, Tomoya;Shoji, Atsusi;Sawai, Hiroaki
以吖啶酮衍生物为荧光标记物，在C5位引入一个末端氨基修饰的胸苷核苷酸，制备了一个带有吖啶酮的胸苷类似物的三磷酸。在PCR过程中使用KOD Dash DNA聚合酶将吖啶酮标记的核苷酸酶促掺入DNA中。此外，我们通过合成后修饰将吖啶酮衍生物引入到氨基修饰的DNA中，并研究了它们的荧光性质和杂交能力。这种带有吖啶酮的荧光标记DNA可作为DNA探针。
Triphosphate of a thymidine analogue bearing acridone was prepared from a modified thymidine nucleotide with a terminal amino group at C5 position and an acridone derivative as a new fluorecsent-tagged nucleotide. The acridone-tagged nucleotide was incorporated into DNA enzymatically during PCR using KOD Dash DNA polymerase. Further, we introduced the acridone derivative into an amino-modified DNA chemically by post-synthetic modification, and investigated their fluorescence properties and hybridization ability. The new fluorescent-labeled DNA bearing acridone will be useful as a DNA probe.