Genetic variation on the NFKB1 genes associates with the outcomes of HCV infection among Chinese Han population
Genetic variation on the NFKB1 genes associates with the outcomes of HCV infection among Chinese Han population
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NFKB1基因遗传变异与中国汉族人群HCV感染结果相关
DOI：
10.1016/j.meegid.2018.07.031
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发表时间：
2018-11-01
影响因子：
3.2
通讯作者：
Yue, Ming
中科院分区：
文献类型：
--
作者：
Fan, Hao-zhi;Huang, Peng;Yue, Ming
目的：为探讨核因子-κ B1（NFKB 1）基因多态性与丙型肝炎病毒（HCV）感染的相关性，采用病例对照研究方法，选取984例HCV未感染者、221例HCV自发清除者和456例HCV持续感染者作为研究对象。使用ABI TaqMan等位基因辨别测定对rs 28362491和rs72696119进行基因分型。结果：rs 28362491位点多态性在3组人群中的频率差异有统计学意义（P < 0.001）。在逻辑回归分析中，rs 28362491-ATTG缺失（D）与主要型rs 28362491-ATTG插入（I）相比，与HCV感染风险显著增加相关。（显性模型：校正OR = 1.332，95%CI = 1.059-1.674，P = 0.014;加性模型：校正后OR = 1.181，95%CI = 1.021-1.367，P = 0.025）。结论：NFKB 1 rs 28362491-D等位基因与中国汉族人群HCV感染易感性相关。
Aim: To investigate whether nuclear factor-kappa B1 (NFKB1) gene polymorphisms are associated with the outcomes of hepatitis C virus (HCV) infection in a Chinese high-risk population.Methods: In this case-control study, 984 HCV-uninfected controls, 221 infected individuals with spontaneous HCV clearance, and 456 with persistent HCV infection were enrolled. Rs28362491 and rs72696119 were genotyped using the ABI TaqMan allelic discrimination assay. The functional annotation of the identified single nucleotide polymorphisms (SNPs) were further evaluated by bioinformatics analysis.Results: Significant differences were observed among the three groups (P < 0.001) in terms of the frequency of rs28362491 SNP. In logistic regression analysis, rs28362491-ATTG deleted (D) was associated with a significantly increased risk of HCV infection compared to the major-type rs28362491-ATTG inserted (I) (dominant model: adjusted OR = 1.332, 95% CI = 1.059-1.674, P = 0.014; additive model: adjusted OR = 1.181, 95% CI = 1.021-1.367, P = 0.025), after adjusting for age, gender, and route of infection. Based on the in silico prediction, the RegulomeDB score for SNP rs28362491 was 3a, indicating that it can potentially regulate the transcription and expression of NFKB1 gene.Conclusion: NFKB1 rs28362491-D allele was functionally associated with the increased risk of susceptibility to HCV infection in the Chinese Han population.