Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach.
Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach.
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DOI：
10.1021/acs.jproteome.0c00596
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发表时间：
2020-11
影响因子：
4.4
通讯作者：
Huanchao Zeng;Yiru Qin;Er-xia Du;Qiulan Wei;Ying Li;Danyan Huang;Guirong Wang;J. Veenstra
中科院分区：
文献类型：
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作者：
Huanchao Zeng;Yiru Qin;Er-xia Du;Qiulan Wei;Ying Li;Danyan Huang;Guirong Wang;J. Veenstra
美洲大蠊（Periplaneta americana）作为一种典型的半变态昆虫，同时也是一种分布于全球的入侵性城市害虫，在基础研究和应用研究方面都备受关注。以往对美洲大蠊神经肽的鉴定研究主要基于生化分离和分子克隆技术。在本研究中，采用了基于基因组学和肽组学相结合的综合方法来鉴定该昆虫的神经肽。首先，通过对美洲大蠊基因组和转录组的计算机分析，预测出67个保守的神经肽或神经激素前体基因。接着，对来自四种不同组织（中枢神经系统、心侧体和咽侧体复合体、中肠以及雄性附腺）的肽提取物进行大规模肽组学分析，从而鉴定出35种保守（预测出的）神经肽以及一种潜在的（新型）神经肽。后续实验揭示了两种保守神经肽（咽侧体抑制素B和短神经肽F）和一种新型神经肽（PaOGS36577）的组织分布、性别差异以及发育模式。我们的研究展示了一个全面的神经肽组以及详细的时空分布模式，为今后美洲大蠊神经肽的功能研究奠定了坚实基础（相关数据可通过ProteomeXchange获取，标识符为PXD021660）。
As a model hemimetabolous insect species and an invasive urban pest that is globally distributed, the American cockroach, Periplaneta americana, is of great interest in both basic and applied research. Previous studies on P. americana neuropeptide identification have been based on biochemical isolation and molecular cloning. In the present study, an integrated approach of genomics- and peptidomics-based discovery was performed for neuropeptide identification in this insect species. First, 67 conserved neuropeptide or neurohormone precursor genes were predicted via an in silico analysis of the P. americana genome and transcriptome. Using a large-scale peptidomic analysis of peptide extracts from four different tissues (the central nervous system, corpora cardiac and corpora allata complex, midgut, and male accessory gland), 35 conserved (predicted) neuropeptides and a potential (novel) neuropeptide were then identified. Subsequent experiments revealed the tissue distribution, sex difference, and developmental patterns of two conserved neuropeptides (allatostatin B and short neuropeptide F) and a novel neuropeptide (PaOGS36577). Our study shows a comprehensive neuropeptidome and detailed spatiotemporal distribution patterns, providing a solid basis for future functional studies of neuropeptides in the American cockroach (data are available via ProteomeXchange with identifier PXD021660).