Suppression of tomato SlNAC1 transcription factor delays fruit ripening
Suppression of tomato SlNAC1 transcription factor delays fruit ripening
复制标题
抑制番茄SlNAC1转录因子延迟果实成熟
DOI：
10.1016/j.jplph.2016.01.014
复制
发表时间：
2016-04-01
影响因子：
4.3
通讯作者：
Meng, Qingwei
中科院分区：
文献类型：
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作者：
Meng, Chen;Yang, Dongyue;Meng, Qingwei
果实成熟是一个复杂的过程，涉及许多生理生化变化，包括乙烯，类胡萝卜素和细胞壁代谢。番茄（Solanum lycopersicum）具有许多优良的遗传特性，是果实发育和成熟的研究模式。在这项研究中，SlNAC 1被克隆。为了进一步研究SlNAC 1的功能，构建了反义表达载体，并将其转入番茄中。结果表明，AS果实后熟延迟，完全成熟时果实外观红色加深。完全成熟的AS水果也产生了更高的总类胡萝卜素和番茄红素含量比野生型（WT）线。AS果实乙烯产生延迟，但发生在更高的程度比WT果实。AS果实的软化比WT果实慢。AS-4果实内源脱落酸（阿坝）含量低于WT。外源阿坝促进了AS果实的软化。此外，AS果实表现出番茄红素和乙烯生物合成相关基因的表达上调，而细胞壁代谢和阿坝合成相关基因的表达下调。因此，SlNAC 1可能与果实成熟有关。(c)2016 Elsevier GmbH. All rights reserved.
Fruit ripening is a complex process involving many physiological and biochemical changes, including those for ethylene, carotenoid, and cell wall metabolism. Tomato (Solanum lycopersicum) serves as a research model for fruit development and ripening because it possesses numerous favorable genetic features. In this study, SlNAC1 was cloned. An antisense (AS) vector was constructed and transferred to tomato to further explore the function of SlNAC1. The results showed that AS fruits exhibited delayed ripening and a deeper red appearance when these fruits were fully ripened. Fully ripened AS fruits also produced higher total carotenoid and lycopene contents than those of the wild-type (WT) line. Ethylene production of AS fruits was delayed but occurred to a higher extent than that of WT fruits. The softening of AS fruits was slower than that of WT fruits. Endogenous abscisic acid (ABA) level in AS-4 fruits was lower than that in WT fruits. Exogenous ABA accelerated the softening of AS fruits. Furthermore, AS fruits demonstrated up-regulated expression of genes related to lycopene and ethylene biosynthesis but down-regulated expression of genes related to cell wall metabolism and ABA synthesis. Therefore, SlNAC1 is likely implicated in fruit ripening. (c) 2016 Elsevier GmbH. All rights reserved.