How females of the rotifer Asplanchna brightwelli swim in darkness and light: an automated tracking study
How females of the rotifer Asplanchna brightwelli swim in darkness and light: an automated tracking study
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雌性轮虫 Asplanchna Brightwelli 如何在黑暗和光明中游泳：一项自动跟踪研究
DOI：
10.1007/bf00025827
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发表时间：
1993
期刊：
影响因子：
2.6
通讯作者：
P. Clément
中科院分区：
文献类型：
--
作者：
P. Mimouni;A. Luciani;P. Clément
这项工作探讨了黑暗的影响，对雌性轮虫的游泳行为的Asplanchna brightwelli。将雌性动物置于平底皿中，在白色光（WL，5000 µW cm−2）和红外光（IR，155 µW cm−2）下交替拍摄，每只雌性动物总共连续拍摄8次，每次15分钟。使用自动跟踪系统来描述每只雌性动物的游泳路径：每秒25个动物重心的x和y坐标，在512 × 512像素的离散空间中。然后计算表征女性游泳性能的指标：线速度，角速度和轨道的其他角参数。用主成分分析法区分WL航迹和IR航迹的游泳特征。雌性在黑暗中比在光明中游得更慢，转身更多。这些结果表明，除了积极的趋光反应外，还有一种趋光效应，它增加了动物在光中的分散度。
This work explores the effect of darkness on the swimming behaviour of females of the rotifer Asplanchna brightwelli. Females were filmed in flat dishes alternately in white light (WL, 5000 µW cm−2) and in infra-red light (IR, 155 µW cm−2), each female for a total of eight successive periods of fifteen minutes per period. An automated tracking system was used to describe the swimming path of each female: twenty five x and y coordinates of the center of gravity of the animal per second, in a discrete space of 512 × 512 pixels. Indices characterizing the swimming performances of the females were then calculated: linear speed, angular speed and other angular parameters of the tracks. A Principal Component Analysis of swimming characteristics discriminated between WL tracks and IR tracks. Females swam slower and turned more in darkness than in light. These results show that beside a positive phototactic response, there is a photokinesis which increases the dispersion of animals in the light.