Subsecond multichannel magnetic control of select neural circuits in freely moving flies
Subsecond multichannel magnetic control of select neural circuits in freely moving flies
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DOI：
10.1038/s41563-022-01281-7
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发表时间：
2022-06-27
期刊：
影响因子：
41.2
通讯作者：
Robinson，Jacob T.
中科院分区：
文献类型：
--
作者：
Sebesta，Charles;Torres Hinojosa，Daniel;Robinson，Jacob T.
基因靶向细胞的精确定时激活是研究神经回路和控制基于细胞的疗法的有力工具。利用磁性纳米颗粒加热温度敏感离子通道，对细胞活动进行磁性控制，或称“磁遗传学”，能够远程、非侵入性地激活神经元，用于深层组织应用和自由行为的动物研究。然而，热磁遗传学的体内响应时间目前为数十秒，这阻止了神经活动的精确时间调制。此外，磁遗传学还没有实现体内多路刺激不同的神经元组。在这里，我们通过将磁性纳米颗粒与速率敏感的温度感受器（TRPA 1-A）相结合，在果蝇中产生亚秒级的行为反应。此外，通过调整磁性纳米颗粒以响应不同的磁场强度和频率，我们实现了亚秒级多通道刺激。这些结果使磁遗传学更接近光遗传学的时间分辨率和多路复用刺激，同时保持只有通过磁控制才可能实现的最小侵入性和深层组织刺激。
Precisely timed activation of genetically targeted cells is a powerful tool for the study of neural circuits and control of cell-based therapies. Magnetic control of cell activity, or ‘magnetogenetics’, using magnetic nanoparticle heating of temperature-sensitive ion channels enables remote, non-invasive activation of neurons for deep-tissue applications and freely behaving animal studies. However, the in vivo response time of thermal magnetogenetics is currently tens of seconds, which prevents precise temporal modulation of neural activity. Moreover, magnetogenetics has yet to achieve in vivo multiplexed stimulation of different groups of neurons. Here we produce subsecond behavioural responses inDrosophila melanogasterby combining magnetic nanoparticles with a rate-sensitive thermoreceptor (TRPA1-A). Furthermore, by tuning magnetic nanoparticles to respond to different magnetic field strengths and frequencies, we achieve subsecond, multichannel stimulation. These results bring magnetogenetics closer to the temporal resolution and multiplexed stimulation possible with optogenetics while maintaining the minimal invasiveness and deep-tissue stimulation possible only by magnetic control.