Alignment of Sfi I sites with the Not I restriction map of Schizosaccharomyces pombe genome
Alignment of Sfi I sites with the Not I restriction map of Schizosaccharomyces pombe genome
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DOI：
10.1093/nar/19.22.6289
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发表时间：
1991-11-25
影响因子：
14.9
通讯作者：
Smith, Cassandra L.
中科院分区：
文献类型：
--
作者：
Fan, Jian-Bing;Grothues, Dietmar;Smith, Cassandra L.
将裂殖酵母裂殖酵母基因组的 Sfi I 限制性图谱与 Not I 限制性图谱进行比对。粟酒裂殖酵母基因组中有 16 个 Sfi I 位点。三个 Sfi I 位点位于第三号染色体上，该染色体缺乏 Not I 位点。根据 Sfi I 片段大小计算的整个基因组和单个染色体的大小与根据 Not I 片段大小计算的一致。 Sfi I 图谱提供了粟酒裂殖酵母基因组的更详细的物理特征，并进一步验证了粟酒裂殖酵母染色体 DNA 作为大小标准的使用。这些图谱可以检测所有三个染色体上的多态性。
A Sfi I restriction map of the fission yeast Schizosaccharomyces pombe genome was aligned with the Not I restriction map. There are 16 Sfi I sites in the S. pombe genome. Three Sfi I sites are on chromosome III which is devoid of Not I sites. The sizes of the entire genome and individual chromosomes, calculated from the Sfi I fragment sizes, are consistent with that calculated from the Not I fragment sizes. The Sfi I map provides greater physical characterization of the S. pombe genome and further validates the use of S. pombe chromosomal DNA as size standard. These maps have allowed detection of polymorphism on all three chromosomes.